Acute damage to the blood–brain barrier and perineuronal net integrity in a clinically-relevant rat model of traumatic brain injury. Issue 16 (4th November 2020)

• Record Type: Journal Article
• Authors: Vita, Sydney M.; Grayson, Bernadette E.; Grill, Raymond J.
• Is Part Of: NeuroReport. Volume 31:Issue 16(2020)
• Publication Date: 2020-11-04
• Subjects: blood–brain barrier -- diffuse injury -- traumatic brain injury -- perineuronal nets; Neurosciences -- Periodicals; Nervous system -- Periodicals; Neurophysiology -- Periodicals; Nervous System Diseases -- Periodicals; Nervous System Physiological Phenomena -- Periodicals; Neurosciences -- Periodicals; 616.805
• DOI: 10.1097/WNR.0000000000001531
• Languages: English
• ISSNs: 0959-4965

Abstract

Abstract : Closed-head, frontal impacts in which the brain undergoes both lateral and rotational acceleration comprise the majority of human traumatic brain injury (TBI). Here, we utilize a clinically relevant model to examine the effects of a single concussion on aspects of brain integrity: the blood–brain barrier, the perineuronal nets (PNNs), and diffuse axonal injury. Adult, male Sprague–Dawley rats received either a frontal, closed-head concussive TBI, or no injury and were evaluated at 1- or 7-day post-injury. Using immunolabeling for albumin, we observed a significant increase in the permeability of the blood–brain barrier at 1-, but not 7-day post-injury. Breakdown of the PNN, as measured by the binding of wisteria floribunda, was observed at 1-day post-injury in the dorsal, lateral, and ventral cortices. This difference was resolved at 7-day. Finally, axonal injury was identified at both 1- and 7-day post-injury. This preclinical model of closed-head, frontal TBI presents a useful tool with which to understand better the acute pathophysiology of a single, frontal TBI.