MiR-181a-2* expression is different amongst carcinomas from the colorectal serrated route. (30th November 2019)

• Record Type: Journal Article
• Authors: Kondelova, Alexandra; Alburquerque-González, Begoña; Vychytilova-Faltejskova, Petra; García-Solano, José; Prochazka, Vladimir; Kala, Zdenek; Pérez, Fernando; Slaby, Ondrej; Conesa-Zamora, Pablo
• Is Part Of: Mutagenesis. Volume 35:Number 3(2020)
• Page Start: 233
• Page End: 241
• Publication Date: 2019-11-30
• Subjects: Mutagenesis -- Periodicals; Mutagenicity Tests -- Periodicals; Mutagens -- Periodicals; Mutagenesis; Periodicals; 576.542
• DOI: 10.1093/mutage/gez039
• Languages: English
• ISSNs: 0267-8357

Abstract

Abstract: Serrated adenocarcinoma (SAC) and colorectal carcinomas showing histological and molecular features of high-level of microsatellite instability (hmMSI-H) are both end points of the serrated pathway of colorectal carcinogenesis. Despite common features (right-sided location, CpG island methylation phenotype and BRAF mutation) there are no studies comparing the microRNA (miRNA) expression profiles in SACs and hmMSI-H. The microtranscriptome from 12 SACs and 8 hmMSI-H were analysed using Affymetrix GeneChip miRNA 3.0 arrays and differentially enriched functions involving immune response were observed from this comparison. miR-181a-2* was found significantly more expressed in hmMSI-H than in SAC and higher expression of this miRNA in microsatellite unstable colorectal cancer were corroborated by Real-Time PCR in an extended series (61 SAC, 21 hmMSI-H). An analysis of genes possibly regulated by miR-181a-2* was carried out and, amongst these, an inverse correlation of NAMPT with miR-181a-2* expression was observed, whereas, for TRAF1 and SALL1, additional regulation mechanisms involving CpG island methylation were observed. miR-181a-2* is associated with particular histological and molecular features of colorectal carcinomas within the serrated pathological pathway and might play a role in the immune responses of microsatellite instability carcinomas.