1H-NMR metabolomics response to a realistic diet contamination with the mycotoxin deoxynivalenol: Effect of probiotics supplementation. (April 2020)

• Record Type: Journal Article
• Authors: Alassane-Kpembi, Imourana; Canlet, Cecile; Tremblay-Franco, Marie; Jourdan, Fabien; Chalzaviel, Maxime; Pinton, Philippe; Cossalter, Anne Marie; Achard, Caroline; Castex, Mathieu; Combes, Sylvie; Bracarense, Ana Paula L.; Oswald, Isabelle P.
• Is Part Of: Food and chemical toxicology. Volume 138(2020)
• Publication Date: 2020-04
• Subjects: Mycotoxin -- Saccharomyces cerevisiae boulardii -- Pig -- Metabolomics -- Histology -- Intestine; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2020.111222
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Low-level contamination of food and feed by deoxynivalenol (DON) is unavoidable. We investigated the effects of subclinical treatment with DON, and supplementation with probiotic yeast Saccharomyces cerevisiae boulardii I1079 as a preventive strategy in piglets. Thirty-six animals were randomly assigned to either a control diet, a diet contaminated with DON (3 mg/kg), a diet supplemented with yeast (4 × 10 9 CFU/kg), or a DON-contaminated diet supplemented with yeast, for four weeks. Plasma and tissue samples were collected for biochemical analysis, 1 H-NMR untargeted metabolomics, and histology. DON induced no significant modifications in biochemical parameters. However, lesion scores were higher and metabolomics highlighted alterations of amino acid and 2-oxocarboxylic acid metabolism. Administering yeast affected aminoacyl-tRNA synthesis and amino acid and glycerophospholipid metabolism. Yeast supplementation of piglets exposed to DON prevented histological alterations, and partial least square discriminant analysis emphasised similarity between the metabolic profiles of their plasma and that of the control group. The effect on liver metabolome remained marginal, indicating that the toxicity of the mycotoxin was not eliminated. These findings show that the 1 H-NMR metabolomics profile is a reliable biomarker to assess subclinical exposure to DON, and that supplementation with S. cerevisiae boulardii increases the resilience of piglets to this mycotoxin. Highlights: Subclinical exposure to DON alters the metabolism of amino acid metabolism and 2-oxocarboxylic acid in piglets. Supplementation of Saccharomyces cerevisiae boulardii I1079 to DON-exposed piglets prevents histological alterations and restores plasma metabolic profile. Yeast supplementation to DON-exposed piglets has limited effect on altered liver metabolome. 1 H-NMR metabolomics profile is a good biomarker for subclinical exposure to DON. Supplementation with Saccharomyces cerevisiae boulardii increases piglet resilience to DON.