A fish cytokine related to human IL-3, IL-5, and GM-CSF, induces development of eosinophil/basophil/mast-cell type (EBM) granulocytes. (July 2020)

• Record Type: Journal Article
• Authors: Nishiya, Kohei; Sawada, Mai; Dijkstra, Johannes M.; Miyamae, Jiro; Okano, Masaharu; Katakura, Fumihiko; Moritomo, Tadaaki
• Is Part Of: Developmental and comparative immunology. Volume 108(2020)
• Publication Date: 2020-07
• Subjects: Teleost -- Common carp -- IL-5fam -- IL-3/IL-5/GM-CSF family -- Beta chain common receptor -- Eosinophil/basophil/mast-cell type granulocyte; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2020.103671
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Interleukin-3 (IL-3), IL-5, and granulocyte-macrophage colony-stimulating factor (GM-CSF) are related cytokines that signal through receptors possessing the β common (βc) chain. As a family, these cytokines combine rather non-specific hematopoietic growth factor properties with a special importance for eosinophils, basophils, and mast cells. In fish the cytokines of this family are called IL-5fam, and the present study, using carp, constitutes their first functional analysis. Carp il-5fam expression was enhanced by stimulation with phytohemagglutinin and killed bacteria. Reminiscent of mammalian IL-3/IL-5/GM-CSF family members, recombinant carp IL-5fam (rcIL-5fam) induced activation of transcription factor STAT5 and efficiently promoted proliferation and colony-formation of eosinophil/basophil/mast-cell type (EBM) granulocytes. Upon addition of recombinant carp βc the growth effect of rcIL-5fam was reduced, suggesting βc participation in the signaling route. In summary, despite differences in individual cytokines and cell populations, fish and mammalian IL-3/IL-5/GM-CSF family members share growth factor functions for non-neutrophil granulocytes. Highlights: The IL-5fam gene related to human IL-3, IL-5 and GM-CSF is conserved in primitive teleosts including, e.g. Cypriniformes and Anguilliformes. Carp il-5fam expression was enhanced by stimulation with killed bacteria and phytohemagglutinin. Recombinant carp IL-5fam (rcIL-5fam) could induce activation of transcription factor STAT5. rcIL-5fam promoted proliferation and colony-formation of kidney cells with eosinophil/basophil/mast-cell type (EBM) granulocyte features. Recombinant carp βc reduced the potential of rcIL-5fam to induce colony-formation.