Evaluation of VIDAS® UP Listeria Assay (LPT) for the Detection of Listeria in a Variety of Foods and Environmental Surfaces: First Action 2013.10. (27th November 2019)

• Record Type: Journal Article
• Authors: Crowley, Erin; Bird, Patrick; Flannery, Jonathan; Benzinger, Jr, M Joseph; Fisher, Kiel; Boyle, Megan; Huffman, Travis; Bastin, Ben; Bedinghaus, Paige; Judd, William; Hoang, Thao; Agin, James; Goins, David; Johnson, Ronald L
• Is Part Of: Journal of AOAC International. Volume 97:Number 2(2014)
• Page Start: 431
• Page End: 441
• Publication Date: 2019-11-27
• Subjects: Agricultural chemistry -- Periodicals; Food -- Analysis -- Periodicals; 543
• DOI: 10.5740/jaoacint.13-372
• Languages: English
• ISSNs: 1060-3271

Abstract

Abstract: The VIDAS ® UP Listeria (LPT) is an automated rapid screening enzyme phage-ligand based assay for the detection of Listeria species in human food products and environmental samples. The VIDAS LPT method was compared in a multi-laboratory collaborative study to AOAC Official Method 993.12 Listeria monocytogenes in Milk and Dairy Products reference method following current AOAC guidelines. A total of 14 laboratories participated, representing government and industry, throughout the United States. One matrix, queso fresco (soft Mexican cheese), was analyzed using two different test portion sizes, 25 and 125 g. Samples representing each test portion size were artificially contaminated with Listeria species at three levels, an uninoculatedcontrol level [0 colony-forming units (CFU)/test portion], a low-inoculum level (0.2–2 CFU/test portion), and a high-inoculum level (2–5 CFU/test portion). For this evaluation, 1800 unpaired replicate test portions were analyzed by either the VIDAS LPT or AOAC 993.12. Each inoculation level was analyzed using the Probability of Detection (POD) statistical model. For the low-level inoculated test portions, difference in collaborator POD (dLPOD) valuesof 0.01, (–0.10, 0.13), with 95% confidence intervals, were obtained for both 25 and 125 g test portions. The range of the confidence intervals for dLPOD values for both the 25 and 125 g test portions contains the point 0.0 indicating no statistically significant difference in the number of positive samples detected between the VIDAS LPT and the AOAC methods. In addition to Oxford agar, VIDAS LPT test portions were confirmed using Agar Listeria Ottavani andAgosti (ALOA), a proprietary chromogenic agar for the identification and differentiation of L. monocytogenes and Listeria species. No differences were observed between the two selective agars. The VIDAS LPT method, with the optional ALOA agar confirmation method, was adopted aOfficial First Action status for the detection of Listeria species in a variety offoods and environmental samples.