Determination of Ibuprofen in Human Plasma and Urine by Gas Chromatography/Mass Spectrometry. (27th November 2019)

• Record Type: Journal Article
• Authors: Yilmaz, Bilal; Erdem, Ali Fuat
• Is Part Of: Journal of AOAC International. Volume 97:Number 2(2014)
• Page Start: 415
• Page End: 420
• Publication Date: 2019-11-27
• Subjects: Agricultural chemistry -- Periodicals; Food -- Analysis -- Periodicals; 543
• DOI: 10.5740/jaoacint.11-414
• Languages: English
• ISSNs: 1060-3271

Abstract

Abstract: This paper describes a GC/MS method for the determination of ibuprofen in human plasma and urine. Ibuprofen and internal standard naproxen were extractedfrom plasma and urine by using a liquid–liquid extraction method. Derivatization was carried outusing N -methyl- N -(trimethylsilyl) trifluoroacetamide. Calibration curves were linear over the concentration range of 0.05–5.0 and 0.1–10.0 μg/mL for plasma and urine, respectively. Intraday and interday precision (RSD) values for ibuprofen in plasma and urine were less than 6.31%, and accuracy (relative error) was better than 12.00%. The mean recovery of ibuprofen was 89.53% for plasma and 93.73% for urine. TheLOD was 0.015 and 0.03 μg/mL and the LOQ was 0.05 and 0.1 μg/mL for plasma and urine, respectively. The method was successfully applied to blood samples from three healthy male volunteers who had been given an oral tablet of 600 mg ibuprofen.