Validation of the AgraStrip® Gluten G12 Test Kit. (27th November 2019)
- Record Type:
- Journal Article
- Title:
- Validation of the AgraStrip® Gluten G12 Test Kit. (27th November 2019)
- Main Title:
- Validation of the AgraStrip® Gluten G12 Test Kit
- Authors:
- Radcliffe, Scott
Sutzko, Meredith
Jiang, Zheng
Freitag, Denise
Swoboda, Christy
Frank, Lukas
Rogers, Adrian - Abstract:
- Abstract: Romer Labs ®, Inc. developed an immunochromatographic lateral flow assay for the qualitative detection of gluten in raw ingredients, processed foods, finished food products, and environmental surfaces, using the G12 antibody developed by Belén Morón. The G12 antibody targets a 33-mer peptide which is resistant to enzymatic digestion and heat denaturation, as well as being the fragment of the gliadin protein to which celiac disease sufferers react, making it a reliable analytical marker. This study was performed to validate the AgraStrip ® Gluten G12 assay method under the guidance of the AOAC Performance Tested Methods SM (PTM) program against AOAC Official Method of Analysis SM 2012.01 in rice flour, bread, cookie, ice cream, and chocolate matrixes spiked with either purified gliadin or wheat gluten standard at 0, 3, 8, 15, and 25 ppm concentrations and tested at the 5, 10, and 20 ppm assay thresholds, as well as on environmental surfaces. Stability, robustness, variation, and lot consistency studies were performed by spiking wheat gluten into a rice flour matrix at 0 and 15 ppm concentrations. The AgraStrip Gluten G12 assay was rigorously evaluated during this study and demonstrates its suitability as an AOAC PTM-certified gluten detection method.
- Is Part Of:
- Journal of AOAC International. Volume 97:Number 6(2014)
- Journal:
- Journal of AOAC International
- Issue:
- Volume 97:Number 6(2014)
- Issue Display:
- Volume 97, Issue 6 (2014)
- Year:
- 2014
- Volume:
- 97
- Issue:
- 6
- Issue Sort Value:
- 2014-0097-0006-0000
- Page Start:
- 1638
- Page End:
- 1650
- Publication Date:
- 2019-11-27
- Subjects:
- Agricultural chemistry -- Periodicals
Food -- Analysis -- Periodicals
543 - Journal URLs:
- http://www.oxfordjournals.org/ ↗
https://academic.oup.com/jaoac/ ↗ - DOI:
- 10.5740/jaoacint.14-143 ↗
- Languages:
- English
- ISSNs:
- 1060-3271
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 15156.xml