Assessing Almond and Peanut Allergens Using Commercially Available Immunoanalytical Kits and LC-MS/MS: A Case Study. (23rd November 2019)

• Record Type: Journal Article
• Authors: Daly, Matthew; Ansari, Parisa; Häubl, Georg; Rogers, Adrian; Brunner, Kurt
• Is Part Of: Journal of AOAC International. Volume 101:Number 1(2018)
• Page Start: 96
• Page End: 101
• Publication Date: 2019-11-23
• Subjects: Agricultural chemistry -- Periodicals; Food -- Analysis -- Periodicals; 543
• DOI: 10.5740/jaoacint.17-0398
• Languages: English
• ISSNs: 1060-3271

Abstract

Abstract: With an ever-increasing allergic population and an emerging market for allergen-free foods, accurate detection of allergens in foods has never been more important. Although ELISA-based methods are the most widely used for detection of allergens in food, there is a need for the development of orthogonal approaches. A commercial ELISA detected a relatively high concentration of peanut and almond in an allergen-free product. However, another commercial ELISA declared a low peanut concentration and was negative for almond. Further testing using a commercial almond lateral-flow device confirmed the results from the second ELISA kit and demonstrated that the positive detection of almond was due to cross-reactivity. An MS method was used for final confirmation that the reported results were negative for both almond and peanut.