Determination of Flurbiprofen in Human Plasma by Gas Chromatography with Mass Spectrometry and Its Pharmacokinetics. (27th November 2019)

• Record Type: Journal Article
• Authors: Yilmaz, Bilal; Sahin, Huseyin; Akba, Vedat; Erdem, Ali Fuat
• Is Part Of: Journal of AOAC International. Volume 97:Number 4(2014)
• Page Start: 1061
• Page End: 1066
• Publication Date: 2019-11-27
• Subjects: Agricultural chemistry -- Periodicals; Food -- Analysis -- Periodicals; 543
• DOI: 10.5740/jaoacint.12-041
• Languages: English
• ISSNs: 1060-3271

Abstract

Abstract: This paper describes a GC/MS method for the determination of flurbiprofen in human plasma. Flurbiprofen and internal standard ibuprofen were extracted from plasma by using a liquid–liquid extraction method. Derivatization was carried out using N -Methyl- N -(trimethylsilyl)trifluoroacetamide. The calibration curve was linear between the concentration range of 0.10 and 5.0 μg/mL. Intraday and interday precision values for flurbiprofen in plasma were less than 5.49%, and accuracy (relative error) was better than 5.33%. The extraction recoveries of flurbiprofen from human plasma were between 93.6 and 98.6%. The LOD and LOQ of flurbiprofen were 0.03 and 0.10 μg/mL, respectively. This assay was applied to determine the pharmacokinetic parameters of flurbiprofen in healthy Turkish volunteers who had been given 100 mg of flurbiprofen.