Rapid Simultaneous Determination of Eperisone, Tolperisone, and Tizanidine in Human Serum by Using a MonoSpin® C18 Extraction Column and Liquid Chromatography/Tandem Mass Spectrometry. (27th November 2019)

• Record Type: Journal Article
• Authors: Miura, Naoya; Saito, Takeshi; Taira, Takayuki; Yamagiwa, Takeshi; Morita, Seiji; Inokuchi, Sadaki
• Is Part Of: Journal of AOAC International. Volume 97:Number 6(2014)
• Page Start: 1546
• Page End: 1551
• Publication Date: 2019-11-27
• Subjects: Agricultural chemistry -- Periodicals; Food -- Analysis -- Periodicals; 543
• DOI: 10.5740/jaoacint.13-295
• Languages: English
• ISSNs: 1060-3271

Abstract

Abstract: A method was developed for rapid toxicological analysis of eperisone, tolperisone, and tizanidine in human serum using a MonoSpin ® C18 extraction column and LC/MS/MS. The method was validated for LOD, linearity, precision, and extraction recovery. This method was rapid with an LOD of 0.5 ng/mL, linearity range 1–500.0 ng/mL (r 2 = 0.999), and RSD value below 14.6%. Extraction recovery from the sample was greater than 98.6, 98.8, and 88.5% for eperisone, tolperisone, and tizanidine, respectively. Results showed that combination of the MonoSpin C18 extraction column and LC/MS/MS is a simple and rapid method for the analysis of these three analytes, and a method is described for simultaneous quantitative determination of the analytes in human serum by LC/MS/MS. This method was used to determine the serum levels of eperisone in a patient with eperisone poisoning, and could be successfully applied for screening analyses in clinical cases other than poisoning.