Group Testing for Severe Acute Respiratory Syndrome– Coronavirus 2 to Enable Rapid Scale-up of Testing and Real-Time Surveillance of Incidence. (27th June 2020)

• Record Type: Journal Article
• Authors: Pilcher, Christopher D; Westreich, Daniel; Hudgens, Michael G
• Is Part Of: Journal of infectious diseases. Volume 222:Number 6(2020)
• Page Start: 903
• Page End: 909
• Publication Date: 2020-06-27
• Subjects: SARS-CoV-2 -- COVID-19 -- group testing -- pooled testing -- diagnostic testing -- screening -- surveillance; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiaa378
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: High-throughput molecular testing for severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2) may be enabled by group testing in which pools of specimens are screened, and individual specimens tested only after a pool tests positive. Several laboratories have recently published examples of pooling strategies applied to SARS-CoV-2 specimens, but overall guidance on efficient pooling strategies is lacking. Therefore we developed a model of the efficiency and accuracy of specimen pooling algorithms based on available data on SAR-CoV-2 viral dynamics. For a fixed number of tests, we estimate that programs using group testing could screen 2–20 times as many specimens compared with individual testing, increase the total number of true positive infections identified, and improve the positive predictive value of results. We compare outcomes that may be expected in different testing situations and provide general recommendations for group testing implementation. A free, publicly-available Web calculator is provided to help inform laboratory decisions on SARS-CoV-2 pooling algorithms. Abstract : High-throughput molecular testing for severe acute respiratory syndrome–coronavirus 2 may be enabled by group testing. Group versus individual testing could allow screening of 2–20 times as many specimens per test and increase the positive predictive value of results.