Mass Spectral Characterization and UPLC Quantitation of 3-Deoxyanthocyanidins in Sorghum bicolor Varietals. (23rd November 2019)

• Record Type: Journal Article
• Authors: Stern, Nathan P; Rana, Jatinder; Chandra, Amitabh; Balles, John
• Is Part Of: Journal of AOAC International. Volume 101:Number 1(2018)
• Page Start: 242
• Page End: 248
• Publication Date: 2019-11-23
• Subjects: Agricultural chemistry -- Periodicals; Food -- Analysis -- Periodicals; 543
• DOI: 10.5740/jaoacint.16-0338
• Languages: English
• ISSNs: 1060-3271

Abstract

Abstract: A quantitative ultra-performance LC (UPLC) method was developed and validated to successfully separate, identify, and quantitate the major polyphenolic compounds present in different varieties of sorghum ( Sorghum bicolor ) feedstock. The method was linear from 3.2 to 320 ppm, with an r 2 of 0.99999 when using luteolinidin chloride as the external standard. Method accuracy was determined to be 99.5%, and precision of replicate preparations was less than 1% RSD. Characterization by UPLC-MS determined that the predominant polyphenolic components of the sorghum varietals were 3-deoxyanthocyanidins (3-DXAs). High-throughput screening for 3-DXA identified four unique classes within the sorghum varieties. Certain feedstock varieties have been found to have a high potential to not only be plant-based colorants, but also provide significant amounts of bioactive 3-DXAs, making them of unique interest to the dietary supplement industry.