Acid and heat fastness in microsporidia: How acid fast are acid fast microsporidium?. (21st January 2020)
- Record Type:
- Journal Article
- Title:
- Acid and heat fastness in microsporidia: How acid fast are acid fast microsporidium?. (21st January 2020)
- Main Title:
- Acid and heat fastness in microsporidia: How acid fast are acid fast microsporidium?
- Authors:
- Mittal, Ruchi
Agarwal, Sunil
Muduli, Kalandi Ch
Sahu, Srikant
Das, Sujata - Abstract:
- Abstract: Microsporidia are obligate spore-forming microorganisms with strong resemblance to fungi and can affect almost every organ system in immunocompetent or immunocompromised individuals. Mixed infections are also reported in immunocompromised hosts. Microsporidial spores show marked morphological variations and the small and slender forms can resemble bacilli. Modified Zeihl Neelsen (ZN) stain, cold method demonstrates them as bright red in color, leaving several spores blue or incompletely stained; thus, they are reported as weakly or variably acid fast. Variability in staining results with ZN stain and considering the fact that Mycobacterium tuberculosis, the commoner bug in developing countries is identified by its resistance to stronger acids on ZN staining, authors wished to demonstrate acid and heat fastness in microsporidium using corneal tissue specimens. Microsporidial spores stained bright red in color with conventional ZN stain, demonstrated strong acid fastness, and interestingly the staining results improved on heating. Thus, the authors conclude that they are strongly acid and heat fast and care must be warranted so that they are not misdiagnosed as Mycobacterium or other acid-fast organisms. Careful observation of morphology, battery of special stains, and molecular diagnostics should be advocated for diagnostic confirmation. To the best of the authors' knowledge, this is the first explicit report on acid and heat fastness on microsporidial spores.
- Is Part Of:
- Medical mycology. Volume 58:Number 7(2020)
- Journal:
- Medical mycology
- Issue:
- Volume 58:Number 7(2020)
- Issue Display:
- Volume 58, Issue 7 (2020)
- Year:
- 2020
- Volume:
- 58
- Issue:
- 7
- Issue Sort Value:
- 2020-0058-0007-0000
- Page Start:
- 1010
- Page End:
- 1013
- Publication Date:
- 2020-01-21
- Subjects:
- Microsporidia -- Mycobacterium -- Zeihl Neelsen stain -- acid fast bacilli -- histopathology
Medical mycology -- Periodicals
Veterinary mycology -- Periodicals
Mycology -- Periodicals
Mycoses -- Periodicals
Pathogenic fungi -- Periodicals
616.969005 - Journal URLs:
- http://mmy.oxfordjournals.org/ ↗
http://ukcatalogue.oup.com/ ↗ - DOI:
- 10.1093/mmy/myz137 ↗
- Languages:
- English
- ISSNs:
- 1369-3786
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 5530.168000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 15139.xml