New approaches for metagenome assembly with short reads. (28th February 2019)

• Record Type: Journal Article
• Authors: Ayling, Martin; Clark, Matthew D; Leggett, Richard M
• Is Part Of: Briefings in bioinformatics. Volume 21:Number 2(2020)
• Page Start: 584
• Page End: 594
• Publication Date: 2019-02-28
• Subjects: Metagenomics -- assembly -- algorithms -- sequencing; Genetics -- Data processing -- Periodicals; Molecular biology -- Data processing -- Periodicals; Genomes -- Data processing -- Periodicals; 572.80285
• DOI: 10.1093/bib/bbz020
• Languages: English
• ISSNs: 1467-5463

Abstract

Abstract: In recent years, the use of longer range read data combined with advances in assembly algorithms has stimulated big improvements in the contiguity and quality of genome assemblies. However, these advances have not directly transferred to metagenomic data sets, as assumptions made by the single genome assembly algorithms do not apply when assembling multiple genomes at varying levels of abundance. The development of dedicated assemblers for metagenomic data was a relatively late innovation and for many years, researchers had to make do using tools designed for single genomes. This has changed in the last few years and we have seen the emergence of a new type of tool built using different principles. In this review, we describe the challenges inherent in metagenomic assemblies and compare the different approaches taken by these novel assembly tools.