Interaction between Lactobacillus reuteri and periodontopathogenic bacteria using in vitro and in vivo (G. mellonella) approaches. Issue 8 (26th August 2020)

• Record Type: Journal Article
• Authors: Santos, Thaís Aguiar; Scorzoni, Liliana; Correia, Raquel; Junqueira, Juliana Campos; Anbinder, Ana Lia
• Is Part Of: Pathogens and disease. Volume 78:Issue 8(2020)
• Publication Date: 2020-08-26
• Subjects: periodontal diseases -- probiotics -- Fusobacterium nucleatum -- Aggregatibacter actinomycetemcomitans -- Lactobacillus reuteri -- Galleria mellonella; Medical microbiology -- Periodicals; Pathogenic microorganisms -- Periodicals; Communicable diseases -- Microbiology -- Periodicals; Communicable diseases -- Pathogenesis -- Periodicals; Host-parasite relationships -- Periodicals; Systems biology -- Periodicals; 616.904105
• DOI: 10.1093/femspd/ftaa044
• Languages: English
• ISSNs: 2049-632X

Abstract

ABSTRACT: Periodontitis is a multifactorial inflammatory disease, and the major cause of tooth loss in adults. New therapies have been proposed for its treatment, including the use of probiotics such as Lactobacillus reuteri . The objective of this study was to evaluate the antimicrobial effects of L. reuteri : live, heat-killed and culture filtrate (cell-free supernatant), on periodontopathogenic bacteria ( Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans ) in vitro, as well as the in vivo survival curve, hemocyte density and microbial recovery using Galleria mellonella . For in vitro assays, all preparations reduced colony forming units of F. nucleatum, while only live L. reuteri reduced the growth of A. actinomycetemcomitans . All treatments reduced periodontopathogenic bacteria growth in vivo . The treatment with the supernatant increased the survival of larvae infected with F. nucleatum more than the treatment with live L. reuteri, and none of the treatments altered the survival of A. actinomycetemcomitans -infected larvae. In addition, the treatment with L. reuteri preparations did not alter the hemocyte count of F. nucleatum- and A. actinomycetemcomitans -infected larvae. This study demonstrated that L. reuteri preparations exerted antimicrobial effects and increased the survival of G. mellonella infected by F. nucleatum, although only live L. reuteri was able to reduce the growth of A. actinomycetemcomitans in vitro . Abstract : In vitro antimicrobial effects of L. reuteri against F. nucleatum does not depend on the probiotic viability, but against A. actinomycetemcomitans, it does and probiotic products were superior to live probiotic in increasing F. nucleatum -infected larvae survival.