Clinical Impact of Rapid Species Identification From Positive Blood Cultures With Same-day Phenotypic Antimicrobial Susceptibility Testing on the Management and Outcome of Bloodstream Infections. (16th May 2019)

• Record Type: Journal Article
• Authors: Ehren, Kathrin; Meißner, Arne; Jazmati, Nathalie; Wille, Julia; Jung, Norma; Vehreschild, Jörg Janne; Hellmich, Martin; Seifert, Harald
• Is Part Of: Clinical infectious diseases. Volume 70:Number 7(2020)
• Page Start: 1285
• Page End: 1293
• Publication Date: 2019-05-16
• Subjects: rapid diagnostic tests -- antimicrobial stewardship -- bacteremia -- Accelerate Pheno system; Communicable diseases -- Periodicals; 616.905
• DOI: 10.1093/cid/ciz406
• Languages: English
• ISSNs: 1058-4838

Abstract

Abstract: Background: Timely availability of microbiological results from positive blood cultures is essential to enable early pathogen-directed therapy. The Accelerate Pheno system (ADX) is a novel technology using fluorescence in situ hybridization for rapid species identification (ID) and morphokinetic bacterial analysis for phenotypic antimicrobial susceptibility testing (AST), with promising results. Yet the impact of this technology on clinical management and patient outcome remains unclear. Methods: We conducted a quasiexperimental before-and-after observational study and analyzed 3 groups with different diagnostic and therapeutic pathways following recent integration of ADX: conventional microbiological diagnostics with and without antimicrobial stewardship program (ASP) intervention, and rapid diagnostics (ADX in addition to conventional standard) with ASP intervention. Primary endpoints were time to adequate, to optimal and to step-down antimicrobial therapy. Secondary endpoints were antimicrobial consumption, in-hospital mortality, length of stay (LOS), and the incidence of Clostridioides difficile infection (CDI). Results: Two hundred four patients (conventional diagnostics, n = 64; conventional diagnostics + ASP, n = 68; rapid diagnostics + ASP; n = 72) were evaluated. The use of ADX significantly decreased time from Gram stain to ID (median, 23 vs 2.2 hours, P < .001) and AST (median, 23 vs 7.4 hours, P < .001), from Gram stain to optimal therapy (median, 11 vs 7 hours, P = .024) and to step-down antimicrobial therapy (median, 27.8 vs 12 hours, P = .019). However, groups did not differ in antimicrobial consumption, duration of antimicrobial therapy, mortality, LOS, or incidence of CDI. Conclusions: Use of ADX significantly reduced time to ID and AST as well as time to optimal antimicrobial therapy but did not affect antimicrobial consumption and clinical outcome. Abstract : The Accelerate Pheno system, in combination with antimicrobial stewardship interventions, significantly reduced time to species identification, to antimicrobial susceptibility testing, and to optimal antimicrobial therapy but did not affect antimicrobial consumption and clinical outcome.