PSXIV-32 Oleic acid stimulate the formation of adipocyte-like cells from bovine satellite cells via G Protein Coupled Receptor 43 and CCAAT/Enhancer Binding Protein Beta. (5th December 2019)

• Record Type: Journal Article
• Authors: Kim, Jongkyoo; Chung, Ki Yong; Johnson, Bradley J; Smith, Stephen B
• Is Part Of: Journal of animal science. Volume 97(2019)Supplement 3
• Page Start: 308
• Page End: 309
• Publication Date: 2019-12-05
• Subjects: bovine satellite cells -- gpr43 -- oleic acid; Livestock -- Periodicals; Livestock; Electronic journals; Periodicals; 636.005
• DOI: 10.1093/jas/skz258.623
• Languages: English
• ISSNs: 0021-8812

Abstract

Abstract: Numerous physiological and pathological processes are controlled by free fatty acids (FFAs), which act as a signaling molecule in mammals. We hypothesized that oleic acid (Ole) may stimulate the formation of satellite cell-derived intramuscular adipose tissue. The objective of the current study was to determine the effect of Ole on GPR43 and factors related to the adipogenic differentiation of bovine satellite cells. Bovine satellite cells were isolated from the semimembranosus of two 14-month-old crossbreed steers. The isolated muscle satellite cells were incubated in Dulbecco's Modified Eagle's Medium (DMEM) solution with 10% Fetal Bovine Serum. Upon reaching 80 to 90% confluence, the growth medium was replaced with differentiation medium composed of DMEM and 2% horse serum, 10μg/mL insulin, 10μg/mL hydrocortisone, 10μM ciglitizone, and 1×antibiotic-antimycotic with dose of: 0, 1, 10, 100, or 500 μM of oleic acid (Ole). Addition of Ole on BSC induced transdifferentiation of myogenic lineage into adipocyte-like cells which formed lipid droplets within cells. Use of 100 μM and 500 μM Ole doses tended to result in a greater (P < 0.1) amount of mRNA gene expression of C/EBPβ compared to all other doses. This might suppress myogenic differentiation. Expression of PPARγ was not altered (P > 0.1) by treatment. The addition of 100 μM and 500 μM upregulated (P < 0.05) mRNA gene expression of GPR43 and 100 μM of Ole increased protein level of GPR43 (P < 0.05) and phosphorylated AMPKα (P < 0.05).