292 Differential gene expression analysis for piglets supplied dietary prebiotics and arachidonic acid for gastrointestinal disturbances. (5th December 2019)

• Record Type: Journal Article
• Authors: He, Yuqing; Jacobi, Sheila; Maltecca, Christian; Odle, Jack
• Is Part Of: Journal of animal science. Volume 97(2019)Supplement 3
• Page Start: 122
• Page End: 123
• Publication Date: 2019-12-05
• Subjects: gene expression -- colitis -- prebiotics; Livestock -- Periodicals; Livestock; Electronic journals; Periodicals; 636.005
• DOI: 10.1093/jas/skz258.253
• Languages: English
• ISSNs: 0021-8812

Abstract

Abstract: Gastrointestinal (GI) disturbances cause significant economic losses in the swine industry. Dietary-based approaches have been well applied to control the inflammation and optimize microbial colonization of the GI tract. This study aimed to screen differentially expressed (DE) RNAs in piglets supplied with arachidonic acid (ARA) or prebiotics in an acute dextran sodium sulfate (DSS) colitis model. Suckling pigs (n = 48) from 6 litters were randomly assigned into 4 diet groups: 0.5% ARA, 0.5% ARA + PRE (4g/L galactooligosaccharide +4 g/L polydextrose), 2.5% ARA, and 2.5% ARA + PRE. On day 17–21, half of the pigs (n = 24) were treated with 0.625 g DSS/kg BW to induce colitis. RNA samples (n = 48) were isolated from the mucosal layer of GI tract on day 22 for the gene expression analysis via RNAseq. The GLIMMIX procedure of SAS (v.9.4) was used to fit the statistical models to gene counts, were the main effects and interactions of PRE, ARA, and DSS were fit in the model as fixed effects and litter as random. Genes with FDR < 0.05 were mapped to the KEGG pathway. A total of 133 DE genes (88 up-regulated, 45 down-regulated) were identified in pigs with colitis compared to healthy ones. PRE and ARA affected gene expression differently but with no interaction effect. PRE supplement decreased the total DE genes from 83 (59 up-regulated, 24 down-regulated) to 33 (16 up-regulated and 17 down-regulated) in pigs with colitis compared with healthy ones. A total of 37 DE genes (21 up-regulated, 16 down-regulated), and 49 DE genes (32 up-regulated, 17 down-regulated) were identified in pigs with colitis compared to healthy ones supplied with 0.5% and 2.5% ARA, respectively. DE genes identified in this study are involved in various signaling pathways with supplied prebiotics significantly changing the gene expression in pigs afflicted with colitis.