Effects of sub‐chronic oral caffeine consumption on memory and the hippocampal Akt, GSK‐3β and ERK in mice: Prevention (nonpharmacological) / Nutrition. (7th December 2020)

• Record Type: Journal Article
• Authors: Moosavi, Maryam; Esmaeili, Zahra; SoukhakLari, Roksana; Moezi, Leila; Pirsalami, Fatema; Farrokhi, Majid Reza; Kazerouni, Armaghan
• Is Part Of: Alzheimer's & dementia. Volume 16(2020)Supplement 10
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-12-07
• Subjects: Alzheimer's disease -- Periodicals; Alzheimer Disease -- Periodicals; Dementia -- Periodicals; Démence; Maladie d'Alzheimer; Périodique électronique (Descripteur de forme); Ressource Internet (Descripteur de forme); 616.83
• DOI: 10.1002/alz.044408
• Languages: English
• ISSNs: 1552-5260

Abstract

Abstract: Background: Caffeine, one of the most widely consumed psychoactive substance in the world, has been shown to affect mood, memory, alertness, and cognitive performance. However, the literature about the effects of caffeine intake on memory is equivocal. This study aimed to assess the effect of sub‐chronic oral gavage of caffeine on memory and the phosphorylation levels of hippocampal Akt (protein kinase B), GSK‐3β (Glycogen Synthase Kinase‐3beta) and ERK (extracellular signal‐regulated kinase) in mice. Method: Adult male NMRI mice were administered with sub‐chronic caffeine at the doses of 0.25, 0.5, 0.75 and 1.5 mg/kg/oral gavage for 10 days before training which continued until the retention day of passive avoidance task. Upon completion of the behavioral assessments, the hippocampi were isolated for western blot analysis to detect the phosphorylated and total levels of Akt, GSK‐3β and ERK proteins. Result: The results showed that sub‐chronic caffeine ingestion at the dose of 0.5 mg/kg improves memory in mice. Furthermore, the memory enhancing dose of caffeine (0.5 mg/kg) elevated the phosphorylated to total ratios of hippocampal Akt, GSK‐3β and ERK. Conclusion: This study suggests that sub‐chronic low dose of caffeine improves memory and increases the phosphorylation of hippocampal Akt, GSK‐3β and ERK proteins.