Members of Prevotella Genus Distinctively Modulate Innate Immune and Barrier Functions in a Human Three-Dimensional Endometrial Epithelial Cell Model. (9th June 2020)

• Record Type: Journal Article
• Authors: Ilhan, Zehra Esra; Łaniewski, Paweł; Tonachio, Adriana; Herbst-Kralovetz, Melissa M
• Is Part Of: Journal of infectious diseases. Volume 222:Number 12(2020)
• Page Start: 2082
• Page End: 2092
• Publication Date: 2020-06-09
• Subjects: ascending infection -- bacterial vaginosis -- biofilm -- colonizer -- genital inflammation -- membrane-associated mucin -- epithelial layer -- pathway prediction -- sialidase -- upper reproductive tract infection; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiaa324
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: Background: Prevotella species are commonly isolated from the reproductive tract of women with obstetric/gynecologic health complications. However, contributions of this genus to changes in local microenvironment are not well characterized. Our objective was to evaluate species-specific effects of Prevotella on the human endometrial epithelium. Methods: Thirteen Prevotella strains, originally isolated from the human oral cavity, amniotic fluid, endometrium, or vagina (including women with bacterial vaginosis), were obtained from BEI and ATCC resources. Bacteria were evaluated in silico and in vitro using human endometrial epithelial cells (EEC) grown as monolayers or a 3-dimensional (3D) model. Results: Genomic characterization illustrated metabolic and phylogenetic diversity of Prevotella genus. Among tested species, P. disiens exhibited cytotoxicity. Scanning electron microscopy analysis of the 3D EEC model revealed species-specific colonization patterns and alterations of ultracellular structures. Infection with sialidase-producing P. timonensis resulted in elongated microvilli, and increased MUC3 and MUC4 expression. Infections with Prevotella species, including P. bivia, did not result in significant proinflammatory activation of EEC. Conclusions: Collectively, findings indicate that Prevotella species are metabolically diverse and overall not cytotoxic or overtly inflammatory in EEC; however, these bacteria can form biofilms, alter barrier properties of the endometrial epithelium, and ultimately impact colonization of secondary colonizers. Abstract : Phylogenetically and metabolically diverse Prevotella interacted with human 3D endometrial epithelial cells (EEC) in a species-specific manner. P. disiens was cytotoxic to the EEC. P. timonensis exhibited high sialidase activity altered membrane-associated mucins more than P. bivia and other species.