The Evolutionary Forces Shaping Cis- and Trans-Regulation of Gene Expression within a Population of Outcrossing Plants. (22nd April 2020)

• Record Type: Journal Article
• Authors: Josephs, Emily B; Lee, Young Wha; Wood, Corlett W; Schoen, Daniel J; Wright, Stephen I; Stinchcombe, John R
• Editors: Purugganan, Michael
• Is Part Of: Molecular biology and evolution. Volume 37:Number 8(2020)
• Page Start: 2386
• Page End: 2393
• Publication Date: 2020-04-22
• Subjects: eQTLs -- trans-regulatory variation -- coexpression networks -- population genetics; Molecular biology -- Periodicals; Molecular evolution -- Periodicals; Evolution, Molecular -- Periodicals; Molecular Biology -- Periodicals; 572.8
• DOI: 10.1093/molbev/msaa102
• Languages: English
• ISSNs: 0737-4038

Abstract

Abstract: Understanding the persistence of genetic variation within populations has long been a goal of evolutionary biology. One promising route toward achieving this goal is using population genetic approaches to describe how selection acts on the loci associated with trait variation. Gene expression provides a model trait for addressing the challenge of the maintenance of variation because it can be measured genome-wide without information about how gene expression affects traits. Previous work has shown that loci affecting the expression of nearby genes (local or cis -eQTLs) are under negative selection, but we lack a clear understanding of the selective forces acting on variants that affect the expression of genes in t rans . Here, we identify loci that affect gene expression in trans using genomic and transcriptomic data from one population of the obligately outcrossing plant, Capsella grandiflora . The allele frequencies of trans -eQTLs are consistent with stronger negative selection acting on trans -eQTLs than cis -eQTLs, and stronger negative selection acting on trans -eQTLs associated with the expression of multiple genes. However, despite this general pattern, we still observe the presence of a trans -eQTL at intermediate frequency that affects the expression of a large number of genes in the same coexpression module. Overall, our work highlights the different selective pressures shaping variation in cis- and trans- regulation.