Controlled Human Infection With Bordetella pertussis Induces Asymptomatic, Immunizing Colonization. (28th September 2019)

• Record Type: Journal Article
• Authors: de Graaf, Hans; Ibrahim, Muktar; Hill, Alison R; Gbesemete, Diane; Vaughan, Andrew T; Gorringe, Andrew; Preston, Andrew; Buisman, Annemarie M; Faust, Saul N; Kester, Kent E; Berbers, Guy A M; Diavatopoulos, Dimitri A; Read, Robert C
• Is Part Of: Clinical infectious diseases. Volume 71:Number 2(2020)
• Page Start: 403
• Page End: 411
• Publication Date: 2019-09-28
• Subjects: Bordetella pertussis -- human challenge -- carriage -- mmune response; Communicable diseases -- Periodicals; 616.905
• DOI: 10.1093/cid/ciz840
• Languages: English
• ISSNs: 1058-4838

Abstract

Abstract: Background: Bordetella pertussis is among the leading causes of vaccine-preventable deaths and morbidity globally. Human asymptomatic carriage as a reservoir for community transmission of infections might be a target of future vaccine strategies, but has not been demonstrated. Our objective was to demonstrate that asymptomatic nasopharyngeal carriage of Bordetella pertussis is inducible in humans and to define the microbiological and immunological features of presymptomatic infection. Methods: Healthy subjects aged 18–45 years with an antipertussis toxin immunoglobin G (IgG) concentration of <20 international units/ml were inoculated intranasally with nonattenuated, wild-type Bordetella pertussis strain B1917. Safety, colonization, and shedding were monitored over 17 days in an inpatient facility. Colonization was assessed by culture and quantitative polymerase chain reaction. Azithromycin was administered from Day 14. The inoculum dose was escalated, aiming to colonize at least 70% of participants. Immunological responses were measured. Results: There were 34 participants challenged, in groups of 4 or 5. The dose was gradually escalated from 10 3 colony-forming units (0% colonized) to 10 5 colony-forming units (80% colonized). Minor symptoms were reported in a minority of participants. Azithromycin eradicated colonization in 48 hours in 88% of colonized individuals. Antipertussis toxin IgG seroconversion occurred in 9 out of 19 colonized participants and in none of the participants who were not colonized. Nasal wash was a more sensitive method to detect colonization than pernasal swabs. No shedding of Bordetella pertussis was detected in systematically collected environmental samples. Conclusions: Bordetella pertussis colonization can be deliberately induced and leads to a systemic immune response without causing pertussis symptoms. Clinical Trials Registration: NCT03751514. Abstract : Asymptomatic Bordetella pertussis nasopharyngeal colonization can be induced in adults, resulting in seroconversion in most colonized volunteers, but not in those noncolonized. Nasal wash sampling is more sensitive than pernasal swabbing. Azithromycin clears carriage in most people within 48 hours.