Modulation of the Host Environment by Human Cytomegalovirus with Viral Interleukin 10 in Peripheral Blood. (1st February 2017)

• Record Type: Journal Article
• Authors: Young, Vivian P.; Mariano, Margarette C.; Tu, Carolyn C.; Allaire, Kathryn M.; Avdic, Selmir; Slobedman, Barry; Spencer, Juliet V.
• Is Part Of: Journal of infectious diseases. Volume 215:Number 6(2017:Mar. 15)
• Page Start: 874
• Page End: 882
• Publication Date: 2017-02-01
• Subjects: CMV -- cmvIL-10 -- cytomegalovirus -- interleukin 10 -- vIL-10 -- viral cytokine.; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jix043
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: Background: Human cytomegalovirus (HCMV) is a herpesvirus with both lytic and latent life cycles. Human cytomegalovirus encodes 2 viral cytokines that are orthologs of human cellular interleukin 10 (cIL-10). Both cytomegalovirus interleukin 10 (cmvIL-10) and Latency-associated cytomegalovirus interleukin 10 (LAcmvIL-10) (collectively vIL-10) are expressed during lytic infection and cause immunosuppressive effects that impede virus clearance. LAcmvIL-10 is also expressed during latent infection of myeloid progenitor cells and monocytes and facilitates persistence. Here, we investigated whether vIL-10 could be detected during natural infection. Methods: Plasma from healthy blood donors was tested by enzyme-linked immunosorbent assay for anti-HCMV immunoglobulin G and immunoglobulin M and for cIL-10 and vIL-10 levels using a novel vIL-10 assay that detects cmvIL-10 and LAcmvIL-10, with no cross-reactivity to cIL-10. Results: vIL-10 was evident in HCMV + donors (n = 19 of 26), at levels ranging 31–547 pg/mL. By comparison, cIL-10 was detected at lower levels ranging 3–69 pg/mL. There was a strong correlation between vIL-10 and cIL-10 levels ( P = .01). Antibodies against vIL-10 were also detected and neutralized vIL-10 activity. Conclusions: vIL-10 was detected in peripheral blood of healthy blood donors. These findings suggest that vIL-10 may play a key role in sensing or modifying the host environment during latency and, therefore, may be a potential target for intervention strategies.