Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays. (15th August 2020)

• Record Type: Journal Article
• Authors: Patterson, Edward I; Prince, Tessa; Anderson, Enyia R; Casas-Sanchez, Aitor; Smith, Shirley L; Cansado-Utrilla, Cintia; Solomon, Tom; Griffiths, Michael J; Acosta-Serrano, Álvaro; Turtle, Lance; Hughes, Grant L
• Is Part Of: Journal of infectious diseases. Volume 222:Number 9(2020)
• Page Start: 1462
• Page End: 1467
• Publication Date: 2020-08-15
• Subjects: SARS-Cov-2 -- inactivation -- temperature -- detergents -- methanol -- paraformaldehyde -- Trizol; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiaa507
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: The scientific community has responded to the coronavirus disease 2019 (COVID-19) pandemic by rapidly undertaking research to find effective strategies to reduce the burden of this disease. Encouragingly, researchers from a diverse array of fields are collectively working towards this goal. Research with infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is undertaken in high-containment laboratories; however, it is often desirable to work with samples at lower-containment levels. To facilitate the transfer of infectious samples from high-containment laboratories, we have tested methods commonly used to inactivate virus and prepare the sample for additional experiments. Incubation at 80°C, a range of detergents, Trizol reagents, and UV energies were successful at inactivating a high titer of SARS-CoV-2. Methanol and paraformaldehyde incubation of infected cells also inactivated the virus. These protocols can provide a framework for in-house inactivation of SARS-CoV-2 in other laboratories, ensuring the safe use of samples in lower-containment levels. Abstract : SARS-CoV-2 research requires the safe use of samples in low-containment laboratories. Heat, UV, and chemical reagents inactivated high titers of virus in solution and cells. These methods will facilitate use of once-infectious samples in downstream procedures.