MicroRNA-1271-5p inhibits cell proliferation and enhances radiosensitivity by targeting CDK1 in hepatocellular carcinoma. (27th December 2019)

• Record Type: Journal Article
• Authors: Liu, Hong-Mei; Tan, Hua-Yan; Lin, Yue; Xu, Bei-Ning; Zhao, Wen-Hua; Xie, Yu-An
• Is Part Of: Journal of biochemistry. Volume 167:Number 5(2020)
• Page Start: 513
• Page End: 524
• Publication Date: 2019-12-27
• Subjects: CDK1 -- hepatocellular carcinoma -- miR-1271-5p -- proliferation -- radiosensitivity; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Electronic journals; 572.05
• DOI: 10.1093/jb/mvz114
• Languages: English
• ISSNs: 0021-924X

Abstract

Abstract: This study aims to determine whether miR-1271-5p inhibits cell proliferation and enhances the radiosensitivity by targeting cyclin-dependent kinase 1 (CDK1) in hepatocellular carcinoma (HCC). Its expression levels in the HCC cell lines were significantly lower than those in normal human liver cell line. Bioinformatics analysis indicated CDK1 was a potential target of miR-1271-5p. Dual-Luciferase Reporter Assay confirmed that CDK1 is a direct target gene of miR-1271-5p. With overexpression of miR-1271-5p in SMMC-7721 and HuH-7 cells, cell proliferation was decreased, radiosensitivity was enhanced, cell cycle distribution was altered and the growth of transplanted tumours in nude mice was significantly reduced. miR-1271-5p overexpression enhanced radiosensitivity, which could be reduced by CDK1 overexpression. Overall, our findings suggested that miR-1271-5p inhibits cell proliferation and enhances the radiosensitivity of HCC cell lines by targeting CDK1.