Evaluation of regenerated tracheal cilia function on a collagen-conjugated scaffold in a canine model. (5th September 2020)

• Record Type: Journal Article
• Authors: Ueda, Yuichiro; Sato, Toshihiko; Nakamura, Ryosuke; Tamari, Shigeyuki; Muranishi, Yusuke; Yutaka, Yojiro; Nakamura, Tatsuo; Omori, Koichi; Iwasaki, Akinori; Date, Hiroshi
• Is Part Of: Interactive cardiovascular and thoracic surgery. Volume 31:Number 5(2020)
• Page Start: 644
• Page End: 649
• Publication Date: 2020-09-05
• Subjects: Artificial trachea -- Regenerated cilia -- Ciliary beat frequency -- Ciliary transport functions; Chest -- Surgery -- Periodicals; Cardiovascular system -- Diseases -- Periodicals; 616.1
• DOI: 10.1093/icvts/ivaa167
• Languages: English
• ISSNs: 1569-9293

Abstract

Abstract: OBJECTIVES: It is unclear whether the movement and function of the regenerated cilia on collagen-conjugated artificial trachea are the same as those of normal cilia. This study assessed the ciliary beat frequency (CBF) and ciliary transport functions (CTFs) of regenerated cilia in a canine model. METHODS: A tracheal defect introduced into the anterior portion of the cervical trachea of an adult beagle dog was covered with a collagen-conjugated prosthesis. Two months later, the trachea was harvested along the long axis, both from normal and regenerated regions. The cilia were stained with isothiocyanate-conjugated wheat germ agglutinin, and their movement was monitored with a high-speed camera to analyse CBF and CTF. Four samples each were obtained from the regenerated and normal regions for CBF analysis and 7 samples each were obtained for CTF analysis. RESULTS: The wheat germ agglutinin-stained cells showed well-regulated beats in both the regenerated and normal regions of the trachea. Mean CBF in the regenerated and normal regions did not differ significantly (7.11 ± 0.41 vs 7.14 ± 1.09 Hz; P = 981). By contrast, CTF was significantly lower in the regenerated region than in the normal region (30.0 ± 6.62 vs 7.43 ± 0.58 μm/s; P = 0.005). CONCLUSIONS: Mean CBF in the regenerated and normal regions did not differ significantly at 2 months. The CTF in the regenerated region recovered partially but remained lower than those in the normal region. Methods are needed to improve the CTF of regenerated cilia. Abstract : An artificial trachea, composed of a collagen sponge and a polypropylene framework, has been developed [1–3].