Simultaneous measurement of biochemical phenotypes and gene expression in single cells. Issue 10 (14th April 2020)

• Record Type: Journal Article
• Authors: Richer, Amanda L; Riemondy, Kent A; Hardie, Lakotah; Hesselberth, Jay R
• Is Part Of: Nucleic acids research. Volume 48:Issue 10(2020)
• Page Start: e59
• Page End: e59
• Publication Date: 2020-04-14
• Subjects: Nucleic acids -- Periodicals; Molecular biology -- Periodicals; 572.805
• DOI: 10.1093/nar/gkaa240
• Languages: English
• ISSNs: 0305-1048

Abstract

Abstract: Methods to measure heterogeneity among cells are rapidly transforming our understanding of biology but are currently limited to molecular abundance measurements. We developed an approach to simultaneously measure biochemical activities and mRNA abundance in single cells to understand the heterogeneity of DNA repair activities across thousands of human lymphocytes, identifying known and novel cell-type-specific DNA repair phenotypes.