Integrated quantitative susceptibility and R2* mapping for evaluation of liver fibrosis: An ex vivo feasibility study. (22nd September 2020)

• Record Type: Journal Article
• Authors: Jafari, Ramin; Hectors, Stefanie J.; Koehne de González, Anne K.; Spincemaille, Pascal; Prince, Martin R.; Brittenham, Gary M.; Wang, Yi
• Is Part Of: NMR in biomedicine. Volume 34:Number 1(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-09-22
• Subjects: liver fibrosis -- magnetic resonance imaging -- quantitative susceptibility mapping; Nuclear magnetic resonance -- Periodicals; Magnetic Resonance Spectroscopy -- Periodicals; 574
• DOI: 10.1002/nbm.4412
• Languages: English
• ISSNs: 0952-3480

Abstract

Abstract : To develop a method for noninvasive evaluation of liver fibrosis, we investigated the differential sensitivities of quantitative susceptibility mapping (QSM) and R2 * mapping using corrections for the effects of liver iron. Liver fibrosis is characterized by excessive accumulation of collagen and other extracellular matrix proteins. While collagen increases R2 * relaxation, measures of R2 * for fibrosis are confounded by liver iron, which may be present in the liver over a wide range of concentrations. The diamagnetic collagen contribution to susceptibility values measured by QSM is much less than the contribution of highly paramagnetic iron. In 19 ex vivo liver explants with and without fibrosis, QSM (χ), R2 * and proton density fat fraction (PDFF) maps were constructed from multiecho gradient‐recalled echo (mGRE) sequence acquisition at 3 T. Median parameter values were recorded and differences between the MRI parameters in nonfibrotic vs. advanced fibrotic/cirrhotic samples were evaluated using Mann–Whitney U tests and receiver operating characteristic analyses. Logistic regression with stepwise feature selection was employed to evaluate the utility of combined MRI measurements for detection of fibrosis. Median R2 * increased in fibrotic vs. nonfibrotic liver samples ( P = .041), while differences in χ and PDFF were nonsignificant ( P = .545 and P = .395, respectively). Logistic regression identified the combination of χ and R2 * significant for fibrosis detection (logit [prediction] = −8.45 + 0.23 R2 * − 28.8 χ). For this classifier, a highly significant difference between nonfibrotic vs. advanced fibrotic/cirrhotic samples was observed ( P = .002). The model exhibited an AUC of 0.909 ( P = .003) for detection of advanced fibrosis/cirrhosis, which was substantially higher compared with AUCs of the individual parameters (AUC 0.591–0.784). An integrated QSM and R2 * analysis of mGRE 3 T imaging data is promising for noninvasive diagnostic assessment of liver fibrosis. Abstract : Ex vivo human liver samples with and without fibrosis were analyzed using R2 *, quantitative susceptibility mapping (QSM) and proton density fat fraction. Logistic regression identified the combination of QSM and R2 * as a significant predictor of fibrosis (logit [prediction] = −8.45 + 0.23 R2* ‐ 28.8 QSM). The model prediction exhibited an AUC of 0.909 ( P = .003) for prediction of advanced fibrosis. An integrated QSM and R2 * analysis of mGRE 3 T imaging data is promising for noninvasive diagnostic assessment of liver fibrosis.