Postoperative placement of an anti‐fibrotic poly L‐lactide electrospun fibrous membrane after sinus surgery. Issue 12 (7th October 2020)

• Record Type: Journal Article
• Authors: Zhang, Tao; Wang, Wei; Cheng, Ruoyu; Tang, Ziying; Chen, Zhengming; Cui, Wenguo; Lian, Bijun; Zheng, Hongliang; Tang, Haihong
• Is Part Of: International forum of allergy & rhinology. Volume 10:Issue 12(2020:Dec.)
• Page Start: 1285
• Page End: 1294
• Publication Date: 2020-10-07
• Subjects: chronic rhinosinusitis -- fibrosis -- biomedical engineering -- postoperative complications -- animal experimentation; 617.51005
• DOI: 10.1002/alr.22666
• Languages: English
• ISSNs: 2042-6976

Abstract

Abstract : Background: Endoscopic sinus surgery (ESS) is used to treat chronic rhinosinusitis. However, nasal adhesions often develop postoperatively, triggered by chronic inflammation and local fibrosis. A poly L‐lactide (PLLA) electrospun microfibrous membrane is a functional biodegradable material that can be placed on the wound surface to protect the wound and prevent adhesions. Methods: We divided 24 rabbits randomly into 2 groups, a control operation group (group A) and an operation+PLLA placement group (group B). We investigated the anti‐fibrotic effects of the topical biomaterial after sinus surgery. We placed PLLA fibrous membranes in the sinus cavity of group B rabbits after sinus surgery, and then evaluated changes in the mucosa and in the levels of collagen fibers, interleukin 4 (IL‐4), IL‐8, tumor necrosis factor α (TNF‐α), transforming growth factor β1 (TGF‐β1), α‐smooth muscle actin (α‐SMA), and collagen I (Col I), using morphological and molecular biological methods. Results: PLLA fibrous membranes did not inhibit the synthesis of messenger RNAs (mRNAs) encoding IL‐4, IL‐8, or TNF‐α, or the protein levels, indicating that the membrane did not have an anti‐inflammatory effect. However, the membrane inhibited the synthesis of mRNAs encoding TGF‐β1, α‐SMA, and Col I, and reduced collagen production. Thus, the nanostructured membrane inhibited fibroblast proliferation. Conclusion: The PLLA membrane had anti‐fibrotic effects, and may be used to prevent fibrosis and adhesions after ESS in human patients.