Evaluation of the progeny produced by interspecific hybridization between Camelina sativa and C. microcarpa. (14th February 2020)

• Record Type: Journal Article
• Authors: Tepfer, Mark; Hurel, Aurélie; Tellier, Frédérique; Jenczewski, Eric
• Is Part Of: Annals of botany. Volume 125:Number 6(2020)
• Page Start: 993
• Page End: 1002
• Publication Date: 2020-02-14
• Subjects: Camelina sativa -- Camelina microcarpa -- interspecific outcrossing -- gene flow -- aberrant meiosis -- interspecific hybrid -- aneuploidy; Botany -- Periodicals; 580
• DOI: 10.1093/aob/mcaa026
• Languages: English
• ISSNs: 0305-7364

Abstract

Abstract: Background and aims: Camelina ( Camelina sativa, Brassicaceae) has attracted interest in recent years as a novel oilseed crop, and an increasing number of studies have sought to enhance camelina's yield potential or to modify the composition of its oil. The ability of camelina to cross-hybridize with its wild relative, C. microcarpa, is of interest as a potential source of genetic variability for the crop. Methods: Manual crosses were performed between the crop C. sativa and its wild relative C. microcarpa ; F 1 and F 2 progenies were obtained. Cytology was used to study meiosis in the parents and F 1 s and to evaluate pollen viability. Flow cytometry was used to estimate nuclear DNA amounts and fatty acid methyl ester analysis was used to evaluate the lipid composition of F 3 seeds. Key Results: The F 1 plants obtained by interspecific crossing presented severe abnormalities at meiosis and low pollen viability, and produced very few F 2 seeds. The F 2 s presented diverse phenotypes and in some cases severe developmental abnormalities. Many F 2 s were aneuploid. The F 2 s produced highly variable numbers of F 3 seeds, and certain F 3 seeds presented atypical lipid profiles. Conclusions: Considering the meiotic abnormalities observed and the probability of aneuploidy in the F 2 plants, the C. microcarpa accessions used in this study would be difficult to use as sources of genetic variability for the crop.