In vitro photoacoustic sensing of calcium dynamics with arsenazo III. (10th June 2016)

• Record Type: Journal Article
• Authors: Dana, N; Fowler, R A; Allen, A; Zoldan, J; Suggs, L; Emelianov, S
• Is Part Of: Laser physics letters. Volume 13:Number 7(2016:Jul.)
• Publication Date: 2016-06-10
• Subjects: biomedical -- photoacoustic -- cell dynamics -- calicum ion -- cell functionality; Lasers -- Periodicals; Physics -- Periodicals; 621.366
• DOI: 10.1088/1612-2011/13/7/075603
• Languages: English
• ISSNs: 1612-2011

Abstract

Abstract: Imaging of cellular electric potential via calcium-ion sensitive contrast agents is a useful tool, but current techniques lack sufficient depth penetration. We explore contrast-enhanced photoacoustic (PA) imaging, using Arsenazo III dye, to visualize cardiac myocyte depolarization in vitro . Phantom results show strong linearity of PA signal with dye concentration ( R 2 > 0.95), and agree spectrally with extinction measurements with varying calcium concentration. Cell studies indicate a significant (>100-fold) increase in PA signal for dye-treated cells, as well as a 10-fold increase in peak-to-peak variation during a 30 s window. This suggests contrast-enhanced PA imaging may have sufficient sensitivity and specificity for depth-resolved visualization of tissue depolarization in real-time.