Nucleic acid based tetrahedral framework DNA nanostructures for fibrotic diseases therapy. (September 2020)

• Record Type: Journal Article
• Authors: Zhang, Tianxu; Gao, Yang; Xiao, Dexuan; Zhu, Junyao; Zhou, Mi; Li, Songhang; Zhang, Mei; Lin, Yunfeng; Cai, Xiaoxiao
• Is Part Of: Applied materials today. Volume 20(2020)
• Publication Date: 2020-09
• Subjects: Tetrahedral DNA nanostructures -- Self-assembly -- DNA nanomaterials -- Fibrotic diseases; Materials science -- Periodicals; Materials -- Research -- Periodicals; 620.1105
• DOI: 10.1016/j.apmt.2020.100725
• Languages: English
• ISSNs: 2352-9407

Abstract

Highlights: Novel tetrahedral framework DNA nanostructures (TFNAs) were synthesized and firstly introduced into fibrotic diseases treatment. TFNAs reduced LPS-induced ROS overproduction which is a crucial stimulator for fibrogenesis. TFNAs suppress extracellular matrix (ECM) production and epithelial-mesenchymal transition (EMT) process. This study provides a new prospect for the application of DNA nanomaterials for fibrotic diseases therapy. Abstract: As a type of novel biomaterial, DNA nanomaterial has been widely applied to biomedical area due to its self-assembly properties. Meanwhile, fibrotic diseases are common health problems, which can lead to multiple organs dysfunction and even death if without proper intervention and treatment. Therefore, we aim to develop the nucleic acids based tetrahedral framework DNA nanostructures (TFNAs) for the treatment of fibrotic diseases. Four single-strand DNA were self-assembled to form tetrahedral framework DNA nanostructures and the anti-fibrotic effects of TFNAs were investigated with an in vitro TGF-β1-induced fibrotic model. TFNAs were successfully assembled and showed obvious cellular uptake. After TFNAs treatment, ROS overproduction, fibrosis-related biomolecules expression including collagen I, fibronectin and α-SMA were obviously reduced. DNA-based TFNAs could be promising biomaterials for the treatment of fibrotic diseases. Graphical abstract: Image, graphical abstract