A novel peroxidase/oxidase mimetic Fe‐porphyrin covalent organic framework enhanced the luminol chemiluminescence reaction and its application in glucose sensing. (22nd June 2020)

• Record Type: Journal Article
• Authors: Zhao, Yaxin; Xu, Xiaotong; Ma, Yuyu; Tan, Haonan; Li, Yinhuan
• Is Part Of: Luminescence. Volume 35:Number 8(2020)
• Page Start: 1366
• Page End: 1372
• Publication Date: 2020-06-22
• Subjects: chemiluminescence -- Fe‐porphyrin covalent organic framework -- glucose -- hydrogen peroxide -- oxidase mimetic -- peroxidase mimetic; Luminescence -- Periodicals; Bioluminescence -- Periodicals; Chemiluminescence -- Periodicals; Luminescence -- Periodicals; 535.35
• DOI: 10.1002/bio.3899
• Languages: English
• ISSNs: 1522-7235

Abstract

Abstract: A Fe‐porphyrin covalent organic framework (Fe‐PorCOF) was prepared through a postmodification strategy and characterized using different techniques. Fe‐PorCOF exhibits an inherent peroxidase/oxidase mimetic catalytic activity and sharply accelerates chemiluminescence (CL) reactions between luminol and hydrogen peroxide (H2 O2 ) or dissolved oxygen (O2 ) under alkaline conditions. The catalytic role was attributed to a significant increase in production of reactive oxygen species. Using the imminent peroxidase mimetic catalytic activity of Fe‐PorCOF, a new CL method was developed for determination of H2 O2 over a linear range from 0.01 to 10.0 μmol·L −1 and with a limit of detection of 5.3 nmol·L −1 . The combination of the peroxidase mimetic catalytic activity of Fe‐PorCOF with the catalytic activity of glucose oxidase on glucose oxidation presents a sensitive CL method for glucose assay. The linear range and the detection limit for glucose were 0.05–8.0 μmol·L −1 and 4.0 nmol·L −1, respectively. The practicability of this method was assessed by determination of glucose in human sera. As a peroxidase/oxidase mimetic, Fe‐PorCOF is easy to prepare and exhibits good catalytic efficiency in the luminol reaction. We believe that this strategy will promote the development of a CL field with functional COFs as a catalyst.