Insecticidal and synergistic activity of dsRNAs targeting buprofezin‐specific genes against the small brown planthopper, Laodelphax striatellus. Issue 4 (14th September 2020)

• Record Type: Journal Article
• Authors: Fang, Ying; Park, Min G.; Choi, Jae Y.; Park, Dong H.; Wang, Minghui; Kim, Hyun J.; Kim, Woo J.; Je, Yeon H.
• Other Names: Kim Jae Su guestEditor.
• Is Part Of: Archives of insect biochemistry and physiology. Volume 105:Issue 4(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-09-14
• Subjects: double‐stranded RNA -- insecticidal activity -- Laodelphax striatellus -- RNA interference; Insects -- Physiology -- Periodicals; Insect biochemistry -- Periodicals; 595.701572
• DOI: 10.1002/arch.21739
• Languages: English
• ISSNs: 0739-4462

Abstract

Abstract: The small brown planthopper, Laodelphax striatellus, is a dangerous pest in rice fields. Although buprofezin has been used to control L. striatellus for more than a decade, the occurrence of buprofezin‐resistant L. striatellus has been recently reported. To develop an alternative pest control strategy, comparative transcriptome analysis of buprofezin‐treated and nontreated L. striatellus was performed to screen the buprofezin‐specific target genes for RNA interference (RNAi) application. Among six genes downregulated in the buprofezin‐treated L. striatellus, RNAi‐based silencing of the lipophorin precursor, endocuticle structure glycoprotein, and chitin synthase significantly induced the lethality of L. striatellus in a concentration‐dependent manner. In addition, a cocktail of double‐stranded RNAs against these three genes showed synergistic effects with buprofezin. These results provide RNAi‐based effective approaches to control L. striatellus as well as an efficient method to identify novel target genes for RNAi application. Graphical abstract: Double‐stranded RNA interference against the lipophorin precursor, endocuticle structure glycoprotein, and chitin synthase gene, which were downregulated in buprofezin‐treated Laodelphax striatellus, significantly induced the lethality of L. striatellus and showed synergistic effects with buprofezin. Highlights: RNAi‐based silencing of Lipophorin precursor, endocuticle structure glycoprotein, and chitin synthase genes induced lethality of L. striatellus . A cocktail of dsRNAs against these genes showed synergistic effects with buprofezin.