Dicrocoelium ova can block the induction phase of experimental autoimmune encephalomyelitis. (25th October 2020) - Navi, Zahra
Abstract: Aims: This study aimed at investigating the impact of Dicrocoelium ova on experimental autoimmune encephalomyelitis (EAE) treatment in C57BL6 mice. Methods and Results: Twenty‐eight C57BL/6 mice were assigned into four groups as PBS, prophylaxis (P), treatment1 (T1) and treatment2 (T2). Prior to induction of EAE in prophylaxis group and on days 7 and 18 in T1 and T2 groups, respectively, Dicrocoelium eggs were injected intraperitoneally to each mouse. The clinical score, weight changes and incidence time of EAE were recorded. IFN‐γ and IL‐4 expression is quantified on spleen cells. Also, histopathological study by (H&E) and Toluidine‐Blue (TB), and Luxol Fast Blue (LFB) were performed. The data were analysed using SPSS version 21. Mean disease scores were significantly lower in P and T1 groups than the PBS group ( P = .01). IFN‐γ was lower in P and T1 groups than the PBS group. The highest level of IL‐4 was observed in T1 group. The total number of neuroglia cells of corpus callosum was similar in all groups, but the density increased in T1 group compared to the PBS group ( P = .03). Conclusions: Dicrocoelium eggs have a great potential to stimulate immunomodulation towards treatment of EAE during the initial phase.