A Near‐Infrared Photo‐Switched MicroRNA Amplifier for Precise Photodynamic Therapy of Early‐Stage Cancers. Issue 48 (18th September 2020)

• Record Type: Journal Article
• Authors: Zhang, Yue; Chen, Weiwei; Zhang, Yue; Zhang, Xiaobo; Liu, Ying; Ju, Huangxian
• Is Part Of: Angewandte Chemie international edition. Volume 59:Issue 48(2020)
• Page Start: 21454
• Page End: 21459
• Publication Date: 2020-09-18
• Subjects: microRNA amplifiers -- photocleavage -- photodynamic therapy -- upconversion nanoparticles; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202009263
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Stimuli‐responsive photodynamic therapy (PDT) is a hot topic in precise medicine, but the low abundance of responsive trigger molecules in early‐stage disease limits application. Here we designed an amplifier with multiple upconversion luminances to achieve a near‐infrared photo‐switched cascade reaction triggered by specific microRNA and precise PDT of early‐stage cancers. This amplifier was composed of photo‐caged DNA nanocombs and an upconversion nanoparticle (UCNP) sensitized with IRDye 800CW. The nanocomb was prepared by assembling a photozipper‐protected hairpin and two kinds of hybridizable hairpin probes on a DNA skeleton. Upon 808‐nm light irradiation, the produced UV light cleaved off the photozipper to induce microRNA‐responsive cascade hybridization reaction, activating the photosensitizers linked to different hairpins to generate reactive oxygen species (ROS) under the simultaneously emitted blue light for efficient PDT. Abstract : A NIR photo‐switched miRNA amplifier is proposed for precise photodynamic therapy of early‐stage cancers. The designed photozipper avoids off‐target damage during in vivo delivery and can be controllably cleaved to trigger miRNA‐responsive cascade activation of photosensitizers under UV and blue emissions of UCNPs, which amplifies ROS generation for highly efficient cancer treatment.