Human Bone Marrow Mesenchymal Stem/Stromal Cells Exposed to an Inflammatory Environment Increase the Expression of ICAM-1 and Release Microvesicles Enriched in This Adhesive Molecule: Analysis of the Participation of TNF-α and IFN-γ. (1st December 2020)

• Record Type: Journal Article
• Authors: Montesinos, Juan J.; López-García, Lucero; Cortés-Morales, Víctor A.; Arriaga-Pizano, Lourdes; Valle-Ríos, Ricardo; Fajardo-Orduña, Guadalupe R.; Castro-Manrreza, Marta E.
• Other Names: Stijlemans Benoit Academic Editor.
• Is Part Of: Journal of immunology research. Volume 2020(2020)
• Publication Date: 2020-12-01
• Subjects: Immunology -- Periodicals; Immunology -- Research -- Periodicals; 616.07905
• DOI: 10.1155/2020/8839625
• Languages: English
• ISSNs: 2314-8861

Abstract

Abstract : Bone marrow mesenchymal stem/stromal cells (BM-MSCs) have immunoregulatory capacity; therefore, they have been used in different clinical protocols in which it is necessary to decrease the immune response. This capacity is mainly regulated by TNF- α and IFN- γ, and it has been observed that cell-cell contact, mainly mediated by ICAM-1, is important for MSCs to carry out efficient immunoregulation. Therefore, in the present work, we analyzed the effect of TNF- α alone or in combination with IFN- γ on the expression of ICAM-1. Besides, given the importance of cell contact in the immunoregulatory function of MSCs, we analyzed whether these cells release ICAM-1 + microvesicles (MVs). Our results show for the first time that TNF- α is capable of increasing the early expression of ICAM-1 in human BM-MSCs. Also, we observed that TNF- α and IFN- γ have a synergistic effect on the increase in the expression of ICAM-1. Furthermore, we found that BM-MSCs exposed to an inflammatory environment release MVs enriched in ICAM-1 (MVs-ICAM-1 high ). The knowledge generated in this study will contribute to the improvement of in vitro conditioning protocols that favor the therapeutic effect of these cells or their products.