Effect of multi-functional polyhydroxylated polyhedral oligomeric silsesquioxane (POSS) nanoparticles on the angiogenesis and exosome biogenesis in human umbilical vein endothelial cells (HUVECs). (1st January 2021)

• Record Type: Journal Article
• Authors: Feghhi, Maryam; Rezaie, Jafar; Akbari, Ali; Jabbari, Nasrollah; Jafari, Hessam; Seidi, Farzad; Szafert, Sławomir
• Is Part Of: Materials & design. Volume 197(2021)
• Publication Date: 2021-01-01
• Subjects: POSS nanoparticles -- Angiogenesis -- HUVECs -- Nanomedicine -- Exosome -- Wound healing; Materials -- Periodicals; Engineering design -- Periodicals; Matériaux -- Périodiques; Conception technique -- Périodiques; Electronic journals; 620.11
• DOI: 10.1016/j.matdes.2020.109227
• Languages: English
• ISSNs: 0264-1275

Abstract

Abstract: Angiogenesis, the growth of new vessels, is a vital process for tissue regeneration. Exosomes are nano-sized vesicles releasing from cells that are recognized as important soluble angiogenic factors. Although there are a lot of reports about biomedical applications of polyhedral oligomeric silsesquioxane nanoparticles (POSS NPs), the effect and mechanism of POSS NPs in angiogenesis and wound healing have not been studied until now. In this research, we have synthesized water-soluble multifunctional polyhydroxylated POSS (POH-POSS) and characterized using FT-IR, TEM, 1 H NMR and 29 Si NMR. We hypothesize that incubation of human endothelial cells (HUVECs) with POH-POSS NPs could contribute to the modulation of angiogenesis and exosome biogenesis. As compared to the control group, the survival rate of cells incubated with 300 ppm POH-POSS significantly increased ( P < 0.05). Wound healing and migration rate of cells increased in POH-POSS treated cells ( P < 0.05). Expression of genes involved in angiogenesis (VEGFR-2, Ang-1, Ang-2) and exosomes biogenesis (Alix, CD63, Rab27a, Rab27b) concurrent with miRNA-21 and miRNA-155 expression up-regulated in treated cells ( P < 0.05). Our findings suggested that up-regulated angiogenesis in POH-POSS treated HUVECs is coincided with an increased exosomal secretory pathway, proposing the key role of exosomes in angiogenesis and possible biomedical application of POH-POSS. Graphical abstract: Unlabelled Image Highlights: Water-soluble POSS nanoparticles (POH-POSS) were synthesized through hydrolyses and condensation method. POH-POSS nanoparticles were characterized using FT-IR, TEM, 1 HNMR and 29 SiNMR techniques. POH-POSS treatment increased angiogenic ability of human endothelial cells (HUVECs)