Raman spectra‐based deep learning: A tool to identify microbial contamination. Issue 11 (16th October 2020)

• Record Type: Journal Article
• Authors: Maruthamuthu, Murali K.; Raffiee, Amir Hossein; De Oliveira, Denilson Mendes; Ardekani, Arezoo M.; Verma, Mohit S.
• Is Part Of: MicrobiologyOpen. Volume 9:Issue 11(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-10-16
• Subjects: biologics -- CHO cells -- convolution neural network -- deep learning -- microbial contamination -- process analytical technology; Microbiology -- Periodicals; 579
• DOI: 10.1002/mbo3.1122
• Languages: English
• ISSNs: 2045-8827

Abstract

Abstract: Deep learning has the potential to enhance the output of in‐line, on‐line, and at‐line instrumentation used for process analytical technology in the pharmaceutical industry. Here, we used Raman spectroscopy‐based deep learning strategies to develop a tool for detecting microbial contamination. We built a Raman dataset for microorganisms that are common contaminants in the pharmaceutical industry for Chinese Hamster Ovary (CHO) cells, which are often used in the production of biologics. Using a convolution neural network (CNN), we classified the different samples comprising individual microbes and microbes mixed with CHO cells with an accuracy of 95%–100%. The set of 12 microbes spans across Gram‐positive and Gram‐negative bacteria as well as fungi. We also created an attention map for different microbes and CHO cells to highlight which segments of the Raman spectra contribute the most to help discriminate between different species. This dataset and algorithm provide a route for implementing Raman spectroscopy for detecting microbial contamination in the pharmaceutical industry. Abstract : We use Raman spectroscopy to identify microbial contaminants that are common in the pharmaceutical industry. These contaminants span across Gram‐negative bacteria, Gram‐positive bacteria, and fungi. The use of a convolution neural network achieves identification accuracy in the range of 95%–100%.