Circ‐SLC8A1 regulates osteoporosis through blocking the inhibitory effect of miR‐516b‐5p on AKAP2 expression. (9th September 2020)

• Record Type: Journal Article
• Authors: Lin, Cheng; Zhong, Wenjun; Yan, Wei; Yang, Jinsong; Zheng, Weiwei; Wu, Qin
• Is Part Of: Journal of gene medicine. Volume 22:Number 11(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-09-09
• Subjects: AKAP2 -- circ‐SLC8A1 -- miR‐516b‐5p -- osteoporosis; Genetic transformation -- Periodicals; Gene Transfer -- Periodicals; Gene Therapy -- Periodicals; 616.042
• DOI: 10.1002/jgm.3263
• Languages: English
• ISSNs: 1099-498X

Abstract

Abstract: Background: Osteoporosis is a disease characterized by bone loss, imbalance of bone metabolism and destruction of trabecular microarchitecture. Circular RNAs (circRNAs) have been revealed as important biological regulators in human diseases. The expression characteristics and mechanism of circRNAs in osteoporosis are unclear. Methods: The binding sites of miR‐516b‐5p on circ‐SLC8A1 and AKAP2 mRNA were predicted using circAtlas (http://circatlas.biols.ac.cn ) and miRDB (http://mirdb.org ). Target sites of miR‐516b‐5p on circ‐SLC8A1 and AKAP2 mRNA were confirmed by a dual luciferase assay. The relationship between miR‐516b‐5p and AKAP2 was determined by a quantitative reverse transcriptase‐polymerase chain reaction. Alizarin red S staining and alkaline phosphatase staining were used to observe the level of osteogenic differentiation after transfection. Results: The first six circRNAs captured from the 30 circRNAs with highest expression in the bone marrow were examined in a mouse model of osteoporosis and the expression of circ‐SLC8A1 was found to be significantly reduced in osteoporosis. Circ‐SLC8A1 negatively regulated the expression of miR‐516b‐5p. Overexpression of circ‐SLC8A1 blocked the inhibition of AKAP2 by miR‐516b‐5p. Conclusions: Circ‐SLC8A1 blocks the inhibitory effect of miR‐516b‐5p on the downstream target gene AKAP2 and promotes osteoporosis. The findings of the present might help to provide a new strategy for the treatment of osteoporosis. Abstract : Circ‐SLC8A1 promotes the mineralized nodule formation and calcium deposits of bone marrow mesenchymal stem cells. A high level of circ‐SLC8A1 enhances the levels of ALP, bone morphogenetic protein 4 (BMP4), osteocalcin (BGLAP) and osteopontin‐like protein (SPP1) in bone marrow mesenchymal stem cells. Circ‐SLC8A1 blocks the inhibitory effect of miR‐516b‐5p on AKAP2 expression.