Estradiol protective role in atherogenesis through LDL structure modification. (24th June 2016)

• Record Type: Journal Article
• Authors: Papi, Massimiliano; Brunelli, Roberto; Ciasca, Gabriele; Maiorana, Alessandro; Maulucci, Giuseppe; Palmieri, Valentina; Parasassi, Tiziana; De Spirito, Marco
• Is Part Of: Journal of physics. Volume 49:Number 28(2016)
• Publication Date: 2016-06-24
• Subjects: low density lipoprotein -- small angle x-ray scattering -- apoB-100 -- 17-β-estradiol -- atherosclerosis; Physics -- Periodicals; 530
• DOI: 10.1088/0022-3727/49/28/285402
• Languages: English
• ISSNs: 0022-3727

Abstract

Abstract: Relevant physiological functions are exerted by circulating low density lipoprotein (LDL) as well as eventual pathological processes triggering atherogenesis. Modulation of these functions can well be founded on modifications of LDL structure. Given its large dimension, multicomponent organization and strong interactions between the protein apoB-100 and lipids, determining LDL 3D structure remains a challenge. We propose a novel quantitative physical approach to this complex biological problem. We introduce a three-component model, fitted to small angle x-ray scattering data on LDL maintained in physiological conditions, able to achieve a consistent 3D structure. Unexpected features include three distinct protein domains protruding out of a sphere, quite rough in its surface, where several core lipid areas are exposed. All LDL components are affected by 17- β -estradiol (E2) binding to apoB-100. Mostly one of the three protruding protein domains, dramatically reducing its presence on the surface and with a consequent increase of core lipids' exposure. This result suggests a structural basis for some E2 protecting roles and LDL physiological modifications.