Mueller matrix signature in advanced fluorescence microscopy imaging. (18th January 2017)

• Record Type: Journal Article
• Authors: Mazumder, Nirmal; Qiu, Jianjun; Kao, Fu-Jen; Diaspro, Alberto
• Is Part Of: Journal of optics. Volume 19:Number 2(2017:Feb.)
• Publication Date: 2017-01-18
• Subjects: microscopy -- polarimetric imaging -- fluorescence microscopy -- imaging systems; Optics -- Periodicals; 535.05
• DOI: 10.1088/2040-8986/aa5114
• Languages: English
• ISSNs: 2040-8978

Abstract

Abstract: We have demonstrated the measurement and characterization of the polarization properties of a fluorescence signal using four-channel photon counting based Stokes–Mueller polarization microscopy. Thus, Lu–Chipman decomposition was applied to extract the critical polarization properties such as depolarization, linear retardance and the optical rotation of collagen type I fiber. We observed the spatial distribution of anisotropic and helical molecules of collagen from the reconstructed 2D Mueller images based on the fluorescence signal in a pixel-by-pixel manner.