Cell‐Penetrating Poly(disulfide) Assisted Intracellular Delivery of Mesoporous Silica Nanoparticles for Inhibition of miR‐21 Function and Detection of Subsequent Therapeutic Effects. Issue 32 (21st June 2016)

• Record Type: Journal Article
• Authors: Yu, Changmin; Qian, Linghui; Ge, Jingyan; Fu, Jiaqi; Yuan, Peiyan; Yao, Samantha C. L.; Yao, Shao Q.
• Is Part Of: Angewandte Chemie international edition. Volume 55:Issue 32(2016)
• Page Start: 9272
• Page End: 9276
• Publication Date: 2016-06-21
• Subjects: cell-penetrating poly(disulfide) -- drug delivery -- endocytosis -- nanoparticles -- small-molecule inhibitor; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201602188
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: The design of drug delivery systems capable of minimal endolysosomal trapping, controlled drug release, and real‐time monitoring of drug effect is highly desirable for personalized medicine. Herein, by using mesoporous silica nanoparticles (MSNs) coated with cell‐penetrating poly(disulfide)s and a fluorogenic apoptosis‐detecting peptide (DEVD‐AAN), we have developed a platform that could be uptaken rapidly by mammalian cells via endocytosis‐independent pathways. Subsequent loading of these MSNs with small molecule inhibitors and antisense oligonucleotides resulted in intracellular release of these drugs, leading to combination inhibition of endogenous miR‐21 activities which was immediately detectable by the MSN surface‐coated peptide using two‐photon fluorescence microscopy. Abstract : Combine and detect : Mesoporous silica nanoparticles are coated with cell‐penetrating poly(disulfide)s and a fluorogenic apoptosis‐detecting peptide. The result is a theranostic drug delivery system for endocytosis‐independent delivery/controlled release of combination drugs for the inhibition of endogenous miR‐21 with simultaneous detection of subsequent drug effects by two‐photon fluorescence microscopy.