Characterization of mRNA Profiles of Exosomes from Diverse Forms of M2 Macrophages. (23rd November 2020)

• Record Type: Journal Article
• Authors: Yue, Yuan; Huang, Suiqing; Wu, Zixuan; Wang, Keke; Li, Huayang; Hou, Jian; Huang, Xiaolin; Luo, Li; Liu, Quan; Wu, Zhongkai
• Other Names: Ortega A. Academic Editor.
• Is Part Of: BioMed research international. Volume 2020(2020)
• Publication Date: 2020-11-23
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2020/1585306
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Exosomes transmit certain amounts of molecules to specific recipient cells for intercellular communication. Among these molecules, messenger RNAs (mRNAs) may be delivered and translated into proteins in the recipient cells, and these mRNAs are thought to be critical mediators of exosomal functions. There are three subtypes of M2 macrophages (M2 Ф), M2a Ф, M2b Ф, and M2c Ф, which have different specific functional programs. The aim of the present study was to screen the mRNA profiles in the exosomes of these macrophage subtypes and to analyze the transcriptomic profile features associated with their specific functions. The mRNA contents of the exosomes isolated from the culture supernatants of the M2 Ф subtypes were analyzed and compared using the Illumina HiSeq platform. The results indicated that the exosomes contained particular mRNAs from their source cells and were messengers of cellular functions. Bioinformatics analysis suggested that the exosomal mRNAs from M2b Фs are enriched in the Toll-like receptor (TLR), tumor necrosis factor (TNF), NOD-like receptor (NLR), and NF-kappa B (NF- κ B) signaling pathways. The mRNA profile of exosomes from M2b Ф was distinctly different from that of exosomes from M2a Ф and M2c Ф and was consistent with the M2b Ф cytological characteristic of maintaining a high level of proinflammatory cytokine and regulatory factor production. Therefore, the mRNA profiles revealed several characteristics of the exosomes from diverse forms of M2 Ф. Further functional investigations based on these results may advance the understanding of the physiological roles of exosome-transferred mRNAs in MФ functions.