Molecular characterization, redox regulation, and immune responses of monothiol and dithiol glutaredoxins from disk abalone (Haliotis discus discus). Issue 107 (December 2020)

• Record Type: Journal Article
• Authors: Kim, Gaeun; Omeka, W.K.M.; Liyanage, D.S.; Lee, Jehee
• Is Part Of: Fish & shellfish immunology. Issue 107(2020)Part A
• Page Start: 385
• Page End: 394
• Publication Date: 2020-12
• Subjects: Abalone -- Glutaredoxins -- Redox homeostasis -- Disulfide reduction; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.10.025
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Glutaredoxins (Grxs) are well-known oxidoreductases involved in a wide range of redox activities in organisms. In this study, two invertebrate Grxs (AbGrx1-like and AbGrx2) from disk abalone were identified and characterized in an effort to gain a deeper understanding into their immune and redox regulatory roles. Both AbGrxs share typical thioredoxin/Grx structures. AbGrx1-like and AbGrx2 were identified as monothiol and diothiol Grxs, respectively. AbGrxs were significantly expressed at the egg and 16-cell stage of early abalone development. Although the expression of both AbGrx s demonstrated similar patterns, the expression of AbGrx1-like was higher than AbGrx2 during development stages. In contrast, AbGrx2 expression was significantly higher than that of AbGrx1-like in adult tissues. Highest AbGrx1-like expression was observed in the hepatopancreas and digestive tract, while highest AbGrx2 expression was found in the gills, followed by the mantle, in healthy adult abalone tissues. The highest expression of AbGrx1-like was observed in the gills at 12 h and 6 h post injection (p.i) of Vibrio parahemolyticus and other stimulants, respectively. The highest expression of AbGrx2 in the gills were observed at 120 h, 6 h, 24 h, and 12 h post injection of V. parahaemolyticus, Listeria monocytogenes, Viral hemorrhagic septicemia virus, and Polyinosinic:polycytidylic acid, respectively. AbGrxs possessed significant 2-hydroxyethyl disulfide (HED) and dehydroascorbate (DHA) reduction activity, but AbGrx2 exhibited higher redox activity than AbGrx1-like. Altogether, our results suggest an important role of AbGrx1-like and AbGrx2 in redox homeostasis, as well as in the invertebrate immune defense system. Our findings will aid the development of new disease management strategies for this economically valuable species. Highlights: Grx1-like and Grx2 from Haliotis discus discus were characterized. AbGrxs showed ubiquitous expression in early embryonic development stages of abalone. AbGrx1-like and AbGrx2 exhibit the highest tissue-specific expression in hepatopancreas and gills, respectively. AbGrxs in gills exhibit significant modulation for immune stimulations. Recombinant AbGrxs displayed significant redox activity.