Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum. Issue 107 (December 2020)

• Record Type: Journal Article
• Authors: Xin, Zifan; Yu, Daode; Yang, Bowen; Chen, Lizhu; Hayouka, Zvi; Chen, Xingfu; Gong, Yitong; Dai, Haiyu; Wang, Lei; Zhao, Yancui; Liu, Xiaoli; Yang, Dinglong
• Is Part Of: Fish & shellfish immunology. Issue 107(2020)Part A
• Page Start: 260
• Page End: 268
• Publication Date: 2020-12
• Subjects: Venerupis philippinarum -- C-type lectin -- Molecular characterization -- Immune recognition; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.10.006
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: In the present study, two C-type lectins (designated as VpClec-3 and VpClec-4) were identified and characterized from the manila clam Venerupis philippinarum . Multiple alignment and phylogenetic relationship analysis strongly suggested that VpClec-3 and VpClec-4 belong to the C-type lectin family. In nonstimulated clams, the VpClec-3 transcript was dominantly expressed in the hepatopancreas, while the VpClec-4 transcript was mainly expressed in gill tissues. Both VpClec-3 and VpClec-4 mRNA expression was significantly upregulated following Vibrio anguillarum challenge. Recombinant VpClec-4 (rVpClec-4) was shown to bind lipopolysaccharide (LPS) and glucan in vitro, whereas recombinant VpClec-3 (rVpClec-3) only bound to glucan. In addition, rVpClec-3 and rVpClec-4 displayed broad agglutination activities towards Vibrio harveyi, Vibrio splendidus and V. anguillarum, while no agglutination activities towards Enterobacter cloacae or Aeromonas hydrophila were observed in rVpClec-3. Moreover, hemocyte phagocytosis was significantly enhanced by rVpClec-3 and rVpClec-4. All the results showed that VpClecs function as pattern recognition receptors (PRRs) with distinct recognition spectra and are potentially involved in the innate immune responses of V. philippinarum . Highlights: Two C-type lectins were identified from the manila clam Ruditapes philippinarum. VpClecs are C-type lectins with different PAMPs binding spectrum. VpClecs can agglutinate Gram-positive and Gram-negative bacteria. VpClecs can function as opsonins to prompt phagocytosis against non-self.