Protective immunity of largemouth bass immunized with immersed DNA vaccine against largemouth bass ulcerative syndrome virus. Issue 107 (December 2020)

• Record Type: Journal Article
• Authors: Jia, Yi-Jun; Guo, Zi-Rao; Ma, Rui; Qiu, De-Kui; Wang, Gao-Xue; Zhu, Bin
• Is Part Of: Fish & shellfish immunology. Issue 107(2020)Part A
• Page Start: 269
• Page End: 276
• Publication Date: 2020-12
• Subjects: LBUSV -- Largemouth bass -- Single-walled carbon nanotubes -- DNA vaccine; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.10.018
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: To reduce the largemouth bass ulcer syndrome (LBUSV) aquatic economic losses, it must take effective preventive measures and coping strategies should be urgently investigated. In this research, the effects of a functionalized single-walled carbon nanotubes (SWCNTs) applied as a delivery vehicle for DNA vaccine administration in largemouth bass ( Micropterus Salmoides ) against LBUSV were studied. Our results showed that SWCNTs loaded with DNA vaccine induced a better protection to largemouth bass against LBUSV. We found more than 10 times increase in serum antibody levels, enzyme activities and immune-related genes ( IL-6, IL-8, IFN-γ, IgM and TNF-α ) expression, in the SWCNTs-pcDNA- MCP immunized groups compared with PBS group and the pure SWCNTs group. The survival rates for control group (PBS), pure SWCNTs groups (40 mg L −1 ), four pcDNA- MCP groups (5 mg L −1, 10 mg L −1, 20 mg L −1 and 40 mg L −1 ) and four SWCNTs-pcDNA- MCP groups (5 mg L −1, 10 mg L −1, 20 mg L −1 and 40 mg L −1 ) were 0%, 0%, 0%, 2.77%, 11.11%, 19.44%, 27.78%, 38.89%, 52.78% and 61.11%, respectively. Our results demonstrate that the SWCNTs-DNA vaccine can be used as a new method against LBUSV showing protection following challenge with LBUSV. Highlights: SWCNTs was used as an efficient DNA vaccine vector against LBUSV infection. Immersed DNA vaccine confers strong immunity against LBUSV. SWCNTs enhance the immunological effect of DNA vaccines.