Engineering Nicotiana tabacum for the de novo biosynthesis of DMNT to regulate orientation behavior of the parasitoid wasps Microplitis mediator. Issue 1 (18th September 2020)

• Record Type: Journal Article
• Authors: Liu, Danfeng; Li, Wei; An, Xingkui; Ghasemzadeh, Somayyeh; Huang, Xinzheng; Chen, Jieyin; Kou, Junfeng; Sun, Peiyao; Zhang, Yongjun
• Is Part Of: Pest management science. Volume 77:Issue 1(2021)
• Page Start: 502
• Page End: 509
• Publication Date: 2020-09-18
• Subjects: DMNT biosynthesis -- co‐expression of GhCYP82Ls and GhTPS14 -- transgenic tobacco -- Microplitis mediator -- orientation behavior; Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.6047
• Languages: English
• ISSNs: 1526-498X

Abstract

Abstract: BACKGROUND: ( E )‐4, 8‐dimethylnona‐1, 3, 7‐triene (DMNT), one of the homoterpenes, is thought to contribute to plant indirect defense against insect herbivores. DMNT‐enriched plants have great application potential to regulate insect behavior in the 'push & pull' strategy of pest management. However, de novo biosynthesis of DMNT in plants without a homoterpene metabolic pathway in their wild type is still not achieved, and the role of DMNT played in these plants and their interacted insects remains unclear. RESULTS: Cytochrome P450s and terpene synthases involved in homoterpenes biosynthesis in cotton plants were employed to generate DMNT‐releasing tobacco plants. Single GhTPS 14 transgenic Nicotiana tabacum only emitted ( E )‐nerolidol, the precursor of DMNT. Transgenic tobaccos expressing single GhCYP 82 L s were unable to produce DMNT or TMTT, while DMNT was detected when exogenous ( E )‐nerolidol was added. Compared to wild‐type plants, only co‐expression of GhCYP 82 L s and GhTPS 14 in transgenic tobaccos triggered the constitutive release of single‐component DMNT. Furthermore, DMNT‐emitting transgenic tobacco plants, whether infested with Helicoverpa armigera larvae or not, significantly incited orientation behavior of parasitoid wasps Microplitis mediator . CONCLUSION: Wild type N. tabacum plants have no DMNT metabolic pathway. DMNT could be de novo biosynthesized via co‐expression of GhCYP 82 L s and GhTPS 14. What is more, the parasitoid wasp M. mediator could be recruited by DMNT‐releasing transgenic tobaccos, especially by H. armigera ‐infested transgenic tobaccos, suggesting the potential roles of engineered N. tabacum in regulating the behavioral preference of M. mediator. Abstract : ( E )‐4, 8‐dimethylnona‐1, 3, 7‐triene (DMNT), one of the homoterpenes, plays an essential role in plant indirect defense against insect herbivores, but wild‐type tobacco plants are lack of DMNT metabolic pathway. Cytochrome P450s and terpene synthases involved in homoterpenes biosynthesis in cotton plants were employed to generate DMNT‐releasing tobacco plants. De novo biosynthesis of DMNT in tobacco was achieved only by co‐expression of GhTPS 14 and GhCYP 82 Ls. DMNT‐emitting transgenic tobacco plants significantly excited the orientation behavior of parasitoid wasps Microplitis mediator. Wild‐type tobacco plants are lack of DMNT metabolic pathway. When cytochrome P450s and terpene synthases involved in homoterpenes biosynthesis in cotton plants were employed, DMNT‐releasing transgenic tobacco plants were generated. Microplitis mediator, the parasitic wasps of Helicoverpa armigera, were significantly prefered to DMNT‐releasing tobacco plants. © 2020 Society of Chemical Industry