Characterization of a cell death‐inducing endonuclease‐like venom protein from the parasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae). Issue 1 (6th August 2020)

• Record Type: Journal Article
• Authors: Wang, Jiale; Yan, Zhichao; Xiao, Shan; Wang, Beibei; Fang, Qi; Schlenke, Todd; Ye, Gongyin
• Is Part Of: Pest management science. Volume 77:Issue 1(2021)
• Page Start: 224
• Page End: 233
• Publication Date: 2020-08-06
• Subjects: Pteromalus puparum -- endonuclease -- venom -- hemocyte -- encapsulation -- cell death -- cytotoxicity -- PpENVP; Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.6011
• Languages: English
• ISSNs: 1526-498X

Abstract

Abstract: BACKGROUND: Parasitoid wasps are valuable natural enemies for controlling pests. To ensure successful parasitism, these wasps inject venoms along with their eggs that are deposited either into or on their hosts. Parasitoid venoms regulate host behaviors, development, metabolism and immune responses. Pteromalus puparum is a pupal endoparasitoid that parasitizes a number of butterflies, including the worldwide pest cabbage butterfly, Pieris rapae . Venom from P. puparum has a variety of effects on host hemocytes, including alteration of absolute and relative hemocyte counts, and inhibition of hemocyte spreading and encapsulation. In particular, P. puparum venom causes hemocyte cell death in vivo and in vitro . RESULTS: Using assay‐guided chromatography, a cell death‐inducing venom fraction was identified and defined as P. puparum endonuclease‐like venom protein ( Pp ENVP). It belongs to the DNA/RNA nonspecific endonuclease family, which contains two conserved endonuclease activation sites. We analyzed its expression profiles and demonstrated that Pp ENVP inhibits gene expression in transfected cells relying on two activation sites. However, RNA interference of Pp ENVP did not significantly reduce P. puparum venom cytotoxicity, suggesting that Pp ENVP may not be the sole cytotoxic factor present. CONCLUSION: Our results provide novel insight into the function of the P. puparum venom cocktail and identify a promising insecticide candidate endonuclease that targets insect hemocytes. Abstract : A cell death‐inducing fraction in Pteromalus puparum venom was identified ( Pp ENVP) by combining transcriptomic and proteomic analyses. It inhibits gene expression in transfected cells relying on two endonuclease activation sites. © 2020 Society of Chemical Industry