FRACTAL: Signal amplification of immunofluorescence via cyclic staining of target molecules. Issue 46 (20th November 2020)

• Record Type: Journal Article
• Authors: Cho, Yehlin; Seo, Junyoung; Sim, Yeonbo; Chung, Jinkyoung; Park, Chan E.; Park, Chun Gwon; Kim, Doory; Chang, Jae-Byum
• Is Part Of: Nanoscale. Volume 12:Issue 46(2020)
• Page Start: 23506
• Page End: 23513
• Publication Date: 2020-11-20
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d0nr05800a
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : We demonstrate a novel signal amplification technique that can amplify the signal intensity of immunofluorescence staining via simple cyclic staining of secondary antibodies. Abstract : In this article, we demonstrate fluorescent signal amplification via cyclic staining of target molecules (FRACTAL), a technique that can amplify the signal intensity of immunofluorescence staining more than nine-fold via simple cyclic staining of secondary antibodies. We also show that FRACTAL is compatible with four-color imaging and expansion microscopy imaging.